کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7788126 | 1500628 | 2015 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
O-2â²-Hydroxypropyltrimethyl ammonium chloride chitosan nanoparticles for the delivery of live Newcastle disease vaccine
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A novel complex chitosan derivative, O-2â²-hydroxypropyltrimethyl ammonium chloride chitosan (O-2â²-HACC), was synthesized and used to make nanoparticles as a delivery vehicle for live attenuated Newcastle disease vaccine. We found that O-2â²-HACC had high antimicrobial activity, low toxicity, and a high safety level. Newcastle disease virus (NDV) was then encapsulated in the O-2â²-HACC nanoparticles (NDV/La Sota-O-2â²-HACC-NPs) by the ionic crosslinking method, and the properties of the resulting nanoparticles were determined by transmission electron microscopy, Zeta potential analysis, Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and X-ray diffraction. NDV/La Sota-O-2â²-HACC-NPs had regular spherical morphologies and high stability, with an encapsulation efficiency of 95.68 ± 2.2% and a loading capacity of 58.75 ± 4.03%. An in vitro release assay indicated that release of NDV from NDV/La Sota-O-2â²-HACC-NPs occurred slowly. Specific pathogen-free chickens immunized with NDV/La Sota-O-2â²-HACC-NPs intranasally had much stronger cellular, humoral and mucosal immune responses than did those immunized intramuscularly or with live attenuated Newcastle disease vaccine. NDV/La Sota-O-2â²-HACC-NPs are a novel drug delivery carrier with immense potential in medical applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 130, 5 October 2015, Pages 280-289
Journal: Carbohydrate Polymers - Volume 130, 5 October 2015, Pages 280-289
نویسندگان
Chunxiao Dai, Hong Kang, Wanqiu Yang, Jinyan Sun, Chunlong Liu, Guogang Cheng, Guangyu Rong, Xiaohua Wang, Xin Wang, Zheng Jin, Kai Zhao,