کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7791012 1500646 2014 28 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification, characterization and immunomodulatory effects of Plantago depressa polysaccharides
ترجمه فارسی عنوان
خالص سازی، خصوصیات و اثرات ایمنی سازی پلی ساکارید های دلفریب پلانتاگو
کلمات کلیدی
دلفری پلی ساکارید اسید، پاکسازی، ساختار جزئی، اثرات ایمنی تخفیف،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی
Four purified polysaccharide fractions from seeds of Plantago depressa (PDSP-1, PDSP-2, PDSP-3 and PDSP-4) were obtained by isolation and purification using DEAE-52 cellulose and Sephacryl S-400 HR chromatography. Basic physicochemical properties including molecular weight, chemical composition, FT-IR and glycosidic linkage of these fractions were investigated. They seemed to be homogeneous acidic protein-bound heteropolysaccharides with high molecular weight of over 1000 kDa and contained a lot more β-type glycosidic linkages than α-type. PDSP-3 mainly contained mannose, arabinose and fucose, and the others were rich in arabinose, fucose and galacturonic acid. The immunomodulatory effects of them were assessed by splenocyte proliferation index and production of NO and TNF-α from macrophages. They all showed significant immunomodulatory activities, and PDSP-3 presented the strongest effect. Their observed differences in biological activities were probably due to their structure differences. And monosaccharide compositions, linkage types and molecular weight may affect their immunomodulatory activities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 112, 4 November 2014, Pages 63-72
نویسندگان
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