Effect of chitosan's amino group in adsorption-crosslinking immobilization of lipase enzyme on resin to catalyze biodiesel synthesis

Lipase as biocatalyst is used in biodiesel production but its price is becoming serious problem. Immobilization could improve the ability of enzyme. Immobilization method which gives higher activity and stability is adsorption-crosslinking method. The addition of amino group on supports has proven to stabilize the enzyme. Thus, this research focused on the improvement of lipase immobilization performance by the addition of chitosan which contains amino group. The highest unit activity (24.69 U/g resin) is reached by immobilized lipase on anion-exchange macroporous resin with addition of chitosan on resin directly. This enzyme produces biodiesel with yield of 50.79%.