Production of α-Amylase and β-Glucosidase from Aspergillus niger by solid state fermentation method on biomass waste substrates from rice husk, bagasse and corn cob

Hydrolysis enzymes can be produced from Aspergillus niger using solid state fermentation method. This research aimed to understand the fermentation efficiency of A. niger with different variety of the biomass waste substrates, i.e. rice husk, sugarcane bagasse and corn cob, to produce α-amylase and β-glucosidase. The optimum fermentation time for each substrate was 6 days. The highest activity unit for α-amylase and β-glucosidase were 81.86 U/ml and 95.02 U/ml using substrate corn cob respectively. Activity units were 73.94 U/ml and 82.35 U/ml measured for dry α-amylase and dry β-glucosidase respectively. This dried enzyme was stable for hydrolysis process at 30-50 °C.