کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8129852 | 1523180 | 2018 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of the cytotoxic effects of combined ultrasound, microbubble and nucleoside analog combinations on pancreatic cells in vitro
ترجمه فارسی عنوان
تجزیه و تحلیل اثر سیتوتوکسی ترکیب اولتراسوند، میکروبروی و ترکیب آنالوگهای نوکلئوزیدی بر روی سلولهای پانکراس در آزمایشگاهی
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
فیزیک و نجوم
آکوستیک و فرا صوت
چکیده انگلیسی
Ultrasonically-stimulated microbubbles enhance the therapeutic effects of various chemotherapy drugs. However, the application of ultrasound and microbubbles (USMB) for enhancing the therapeutic effect of nucleoside analogs, which are used as front-line treatments in a range of cancers, and its underlying mechanism is not well understood. This study investigated the effect of gemcitabine, a nucleoside analog drug, in combination with USMB in increasing cell cytotoxicity relative to either treatment alone in BxPC3 pancreatic cancer cells. Cells were sonicated using low frequency (0.5â¯MHz) ultrasound in combination with Definity® microbubbles (1.7% v/v) in the presence of 1â¯ÂµM of gemcitabine for a total of 2â¯h. USMB in combination with gemcitabine decreased cell viability (48â¯h) to 44.7â¯Â±â¯5.2%, 27.7â¯Â±â¯4.3%, and 12.5â¯Â±â¯3.4% with increasing ultrasound peak negative pressures (220, 360, 530â¯kPa) from 84.7â¯Â±â¯3.6%, 54.2â¯Â±â¯3.8%, and 26.8â¯Â±â¯3.0%, respectively, when USMB was applied in the absence of drug. We further confirmed that USMB did not enhance the internalization of 1â¯ÂµM of a radiolabeled nucleoside analog (2-chloroadenosine) at each of the three chosen ultrasound PNPs, determined by radiolabeled scintillation counting. These data suggest that USMB in combination with nucleoside analog drugs leads to an additive effect on cell toxicity and that USMB does not impair transporter-mediated uptake of nucleoside analogs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultrasonics - Volume 89, September 2018, Pages 110-117
Journal: Ultrasonics - Volume 89, September 2018, Pages 110-117
نویسندگان
Julia Mariglia, Shadab Momin, Imogen R. Coe, Raffi Karshafian,