Lipidomics of oxidized polyunsaturated fatty acids

► Polyunsaturated fatty acids oxygenate to eicosanoids, docosanoids, and octadecanoids. ► We performed simultaneous analysis of lipids by liquid chromatography-tandem mass spectrometry. ► Multiple lipid mediators were detected at low picograms per milliliter and in various biological matrices. ► Mediator lipidomics are relevant to biological, nutritional, and pharmacological studies.