کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8292505 | 1536733 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Drug delivery using polyhistidine peptide-modified liposomes that target endogenous lysosome
ترجمه فارسی عنوان
تحویل دارو با استفاده از لیپوزومهای اصلاح شده پپتید پلی هیستیدین که لیزوزوم اندوژن را هدف قرار می دهند
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کلمات کلیدی
DOPCPBSDDSsFBSCLSMMFICPZLSDsGLACPPs - CPP هاalpha-galactosidase A - آلفا گالاکتوزیداز ALysosomal storage diseases - بیماری های ذخیره سازی لیزوزومیminimal essential medium - حداقل وسایل ضروریDioleoyl phosphatidylcholine - دیویلیل فسفاتیدیل کولینfetal bovine serum - سرم جنین گاوDrug delivery system - سیستم تحویل داروDrug delivery systems - سیستم های تحویل داروLysosome - لیزوزومLiposome - لیپوزوم هاMEM - مامانPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریmean fluorescence intensity - میانگین شدت فلورسانسconfocal laser scanning microscopy - میکروسکوپهای اسکن لیزری کانفوکالhistidine - هیستیدینCell-penetrating peptide - پپتید نفوذ سلولیcell-penetrating peptides - پپتیدهای نفوذ سلولیchlorpromazine - کلرپرمازین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Cell-penetrating peptides (CPPs) can deliver payloads into cells by forming complexes with bioactive molecules via covalent or non-covalent bonds. Various CPPs have been applied in CPP-modified liposomes, and their effectiveness is highly regarded in liposomal drug delivery systems (DDSs). Previously, we have reported on the polyhistidine peptide (H16 peptide: HHHHHHHHHHHHHHHH-NH2) as a new CPP. The H16 peptide has a higher cell-penetrating capacity than well-known CPPs and delivers small molecules such as fluorescent dyes, bioactive peptides, and proteins into mammalian cells. However, it is not known whether the H16 peptide can deliver large cargos such as liposomes into cells. To assess the potential of the H16 peptide, in this study, we developed H16 peptide-modified liposomes (H16-Lipo) and evaluated their effectiveness in a liposomal DDS. The H16-Lipo was prepared by inserting a stearyl-H16 peptide into the hydrophobic region of a liposome. The H16-Lipo was internalized into human fibrosarcoma cells via multiple endocytosis pathways and localized to intracellular lysosomes. Based on this result, we used the H16-Lipo as a lysosome-targeting DDS. The H16-Lipo delivered alpha-galactosidase A (GLA), one of the lysosomal enzymes, to intracellular lysosomes and improved the proliferation of GLA-knockdown cells. These results suggest that the H16-Lipo is an effective drug carrier for lysosomal enzymes in a lysosome-targeting DDS. The loss of lysosomal enzymes has been known to induce metabolic disorders, called lysosomal storage diseases (LSDs). Our findings indicate that this combination of the H16 peptide and a liposome is a promising candidate as a DDS for the treatment of LSDs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 501, Issue 3, 27 June 2018, Pages 648-653
Journal: Biochemical and Biophysical Research Communications - Volume 501, Issue 3, 27 June 2018, Pages 648-653
نویسندگان
Taiki Hayashi, Matsumi Shinagawa, Tsuyoshi Kawano, Takashi Iwasaki,