کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8301772 | 1537707 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MicroRNA-192* impairs adipocyte triglyceride storage
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کلمات کلیدی
ALDH3A2SGBSqPCRquantitative reverse transcriptase PCRSCARB1SCDAP2GLUT4Adipogenesis - آدیپوژنزvisceral adipose tissue - بافت چربی درشتTranscriptome - ترانسکریپتومtriglyceride - تریگلیسریدSimpson–Golabi–Behmel syndrome - سندرم سیمپسون-گلابی-بهملbody mass index - شاخص توده بدنBMI - شاخص توده بدنیnon-targeting - غیر هدفگیریVAT - مالیات بر ارزش افزودهObesity - مرض چاقیMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAGlucose transporter type 4 - نوع حمل گلوکز 4adipocyte protein 2 - پروتئین آدیپوسیت 2Perilipin 1 - پریلیپین 1PLIN - کامل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We investigated the expression of miR-192* (miR-192-3p) in the visceral adipose tissue (VAT) of obese subjects and its function in cultured human adipocytes. This miRNA is a 3â² arm derived from the same pre-miRNA as miR-192 (miR-192-5p) implicated in type 2 diabetes, liver disease and cancers, and is predicted to target key genes in lipid metabolism. In morbidly obese subjects undergoing bariatric surgery preceded by a very low calorie diet, miR-192* in VAT correlated negatively (r = â 0.387; p = 0.046) with serum triglyceride (TG) and positively with high-density lipoprotein (HDL) concentration (r = 0.396; p = 0.041). In a less obese patient cohort, the miRNA correlated negatively with the body mass index (r = â 0.537; p = 0.026). To characterize the function of miR-192*, we overexpressed it in cultured adipocytes and analyzed the expression of adipogenic differentiation markers as well as cellular TG content. Reduced TG and expression of the adipocyte marker proteins aP2 (adipocyte protein 2) and perilipin 1 were observed. The function of miR-192* was further investigated by transcriptomic profiling of adipocytes expressing this miRNA, revealing impacts on key lipogenic genes. A number of the mRNA alterations were validated by qPCR. Western analysis confirmed a marked reduction of the lipogenic enzyme SCD (stearoyl coenzyme A desaturase-1), the fatty aldehyde dehydrogenase ALDH3A2 (aldehyde dehydrogenase 3 family member A2) and the high-density lipoprotein receptor SCARB1 (scavenger receptor B, type I). SCD and ALDH3A2 were demonstrated to be direct targets of miR-192*. To conclude, the present data identify miR-192* as a novel controller of adipocyte differentiation and lipid homeostasis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1861, Issue 4, April 2016, Pages 342-351
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1861, Issue 4, April 2016, Pages 342-351
نویسندگان
Raghavendra Mysore, You Zhou, Sanja Sädevirta, Hanna Savolainen-Peltonen, P.A. Nidhina Haridas, Jarkko Soronen, Marja Leivonen, Antti-Pekka Sarin, Pamela Fischer-Posovszky, Martin Wabitsch, Hannele Yki-Järvinen, Vesa M. Olkkonen,