کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8303690 | 1537949 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ras-Raf-MAPK signaling promotes nuclear localization of FOXA transcription factor SGF1 via Ser91 phosphorylation
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کلمات کلیدی
TORC1FOXSRMITRAQLC–MS/MS - LC-MS / MSm/z - m / zMAPK - MAPKisobaric tags for relative and absolute quantitation - برچسب های ایزوباریوم برای مقدار نسبی و مطلقTiO2 - تیتانیوم دی اکسیدforkhead box - جعبه جعبهKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوTitanium dioxide - دی اکسید تیتانیومposterior silk gland - غده خفیف خلفیFoxa - فاکساPhosphorylation - فسفریلاسیونNuclear localization - محلی سازی هسته ایMass-to-charge ratio - نسبت جرم به شارژselected reaction monitoring - نظارت بر واکنش انتخاب شدهtarget of rapamycin complex 1 - هدف از مجموعه رپامایسین 1Gene ontology - هستیشناسی ژنیPSG - پاری سن ژرمنmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenliquid chromatography–tandem mass spectrometry - کروماتوگرافی مایع و اسپکترومتری توده دو طرفه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ras-Raf-MAPK signaling promotes cell proliferation and cell survival. We previously reported that Ras1CA overexpression, specifically in the posterior silk glands (PSGs) of the silkworm Bombyx mori, increased fibroin synthesis and cell size, resulting in improved silk yields. In this study, we compared the iTRAQ-based phosphoproteomic profiles of PSGs from wild-type and Ras1CA-overexpressing silkworms. Silk gland factor 1 (SGF1), a FOXA transcription factor that plays a critical role in activating fibroin gene expression, was identified as a phosphoprotein harboring Ser91 as a potential MAPK phosphorylation site. Ser91 phosphorylation of SGF1 was enhanced by Ras1CA overexpression, and this finding was verified by selected reaction monitoring. Consistently, MAPK activity is well correlated with Ser91 phosphorylation of SGF1 and its nuclear localization in PSG cells during silkworm development. Ras1CA overexpression and treatment with inhibitors of Ras signaling promoted or inhibited SGF1 nuclear localization, respectively; mutation of Ser91 to Ala91 eliminated SGF1 nuclear localization. Moreover, MAPK binds to SGF1 and directly phosphorylates Ser91, demonstrating Ser91 as a MAPK phosphorylation site in SGF1. In conclusion, Ras-Raf-MAPK signaling promotes SGF1 nuclear localization for transactivation via Ser91 phosphorylation in silkworms, showing that FOXA transcription factors are regulated via MAPK phosphorylation in animals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1865, Issue 4, April 2018, Pages 560-571
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research - Volume 1865, Issue 4, April 2018, Pages 560-571
نویسندگان
Li Ma, Kang Li, Yaxin Guo, Xiang Sun, Huimin Deng, Kai Li, Qili Feng, Sheng Li,