کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8305618 | 1538431 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification and characterization of a novel antitumor protein with antioxidant and deoxyribonuclease activity from edible mushroom Pholiota nameko
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کلمات کلیدی
DCFH-DAphosphate buffered saline with tween-204′,6-diamidino-2-phenyl-indolePholiota namekohalf inhibitory concentrationJC-1RIPAHBSSdeoxyribonucleaseDAPIHRSABCAPBSTAAPHIC50BHASRBPBSDPPHPVDFTCA1,1-diphenyl-2-picrylhydrazyl - 1،1-دیفنیل-2-پریکیل هیدرازیل2′,7′-dichlorofluorescin diacetate - 2 '، 7'-dichlorofluorescin diacetate2,2′-azobis (2-amidinopropane) dihydrochloride - 2،2'-azobis (2-آمیدین پروپان) دی هیدروکلرایدDMSO - DMSODNAse - DNAaseRadio immunoprecipitation assay - آزمایش ایمنی ادرار رادیوییAntioxidant - آنتی اکسیدانButylated hydroxy anisole - آنزیم هیدروکسی بوسیلهtrichloroacetic acid - اسید ترشکلراکتیکbicinchoninic acid - بیسینکنینیک اسیدApoptosis - خزان یاختهایDimethyl sulfoxide - دیمتیل سولفواکسیدsulforhodamine B - سولفوردامین بAntitumor - ضد تومورphosphate buffer saline - فسفات بافر شورHydroxyl radical scavenging activity - فعالیت هضم رادیکال هیدروکسیلHanks' balanced salt solution - محلول نمک متعادل هانکسPurification - پاکسازیPropidium iodide - پروتئین یدیدPolyvinylidene fluoride - پلی وینیلیدین فلوراید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A novel antitumor protein from the edible mushroom Pholiota nameko (PNAP) was purified through a two-step chromatographic procedure including SP cation exchange chromatogram and Superdex gel filtration. The approximate molecular weight was demonstrated to be 18.5 kDa by SDS-PAGE and ultracentrifugation analysis and N-terminal sequence was detected as AGRTFIGYNG by Edman degradation. Biochemical characterization showed that it exhibited significant antioxidant activity by effectively scavenging hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals compared to standard antioxidant butylated hydroxy anisole. PNAP had deoxyribonuclease activity with the optimum pH and temperature were 5.0 and 60 °C respectively, as well as it can act on both double-stranded and single-stranded DNA, but preferentially on double-stranded DNA. PNAP displayed antitumor activity against cancer cell lines such as MCF7 and Hela cells. Human breast cancer MCF7 cells treated with PNAP produced typical apoptotic morphological changes including chromatin condensation, accumulation of sub-G1 cells and alternation of mitochondrial permeability. The PNAP induced apoptosis of MCF7 cells entailed loss of mitochondrial membrane potential resulting in release of cytochrome c into cytosol, activation of caspase-9 and caspase-3, which are responsible for the execution of apoptosis, implying intrinsic signal pathway is involved in PNAP induced apoptosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 99, April 2014, Pages 28-37
Journal: Biochimie - Volume 99, April 2014, Pages 28-37
نویسندگان
Yeni Zhang, Zhaokun Liu, Tzi Bun Ng, Zhihui Chen, Wentao Qiao, Fang Liu,