کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8393506 | 1544070 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pseudomonas silesiensis sp. nov. strain A3T isolated from a biological pesticide sewage treatment plant and analysis of the complete genome sequence
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کلمات کلیدی
MIGSDDHPHBPHAMLSACoGPesticide - آفت کشmultilocus sequence analysis - تجزیه و تحلیل توالی multilocusWastewater treatment plant - تصفیه خانه فاضلاب Complete genome sequence - توالی کامل ژنومSoil - خاکclusters of orthologous groups - خوشه های گروه های ارتوقیPseudomonas - سودوموناfatty acid methyl esters - متیل استرهای اسید چربFAME یا fatty acid methyl esters - متیل استرهای اسید چربDNA-DNA hybridization - هیبریداسیون DNA DNApolyhydroxyalkanoate - پلی هیدروکسی آلکینواتPolyhydroxybutyrate - پلی هیدروکسی بوتیرت
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Microorganisms classified in to the Pseudomonas genus are a ubiquitous bacteria inhabiting variety of environmental niches and have been isolated from soil, sediment, water and different parts of higher organisms (plants and animals). Members of this genus are known for their metabolic versatility and are able to utilize different chemical compounds as a source of carbon, nitrogen or phosphorus, which makes them an interesting microorganism for bioremediation or bio-transformation. Moreover, Pseudomonas sp. has been described as a microorganism that can easily adapt to new environmental conditions due to its resistance to the presence of high concentrations of heavy metals or chemical pollution. Here we present the isolation and analysis of Pseudomonas silesiensis sp. nov. strain A3T isolated from peaty soil used in a biological wastewater treatment plant exploited by a pesticide packaging company. Phylogenetic MLSA analysis of 4 housekeeping genes (16S rRNA, gyrB, rpoD and rpoB), complete genome sequence comparison (ANIb, Tetranucleotide identity, digital DDH), FAME analysis, and other biochemical tests indicate the A3T strain (type strain PCM 2856TÂ =Â DSM 103370T) differs significantly from the closest relative species and therefore represents a new species within the Pseudomonas genus. Moreover, bioinformatic analysis of the complete sequenced genome showed that it consists of 6,823,539Â bp with a 59.58Â mol% GÂ +Â C content and does not contain any additional plasmids. Genome annotation predicted the presence of 6066 genes, of which 5875 are coding proteins and 96 are RNA genes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Systematic and Applied Microbiology - Volume 41, Issue 1, January 2018, Pages 13-22
Journal: Systematic and Applied Microbiology - Volume 41, Issue 1, January 2018, Pages 13-22
نویسندگان
MichaÅ A. Kaminski, Ewa M. Furmanczyk, Adam Sobczak, Andrzej Dziembowski, Leszek Lipinski,