Molecular dissection of botulinum neurotoxin reveals interdomain chaperone function

► Botulinum neurotoxin (BoNT) is the causative agent of botulism. ► BoNT is one of the most feared biological weapons of the 21st century. ► BoNT is a modular nanomachine. ► The dynamic interplay between modules underlies both activity and inhibitor sensitivity. ► The BoNT protein-conducting channel is a target for intervention.