کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8415525 | 1545521 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Investigation of conformation change of glycated ovalbumin obtained by Co-60 gamma-ray irradiation under drying treatment
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Irradiation can significantly improve the incorporation of glucose into protein. In this study, the efficacy on conformation change and glycation extent of ovalbumin (OVA) powder was investigated during Co-60 irradiation treatment. Spectrophotometer was used to monitor the conformation and structure change of glycation OVA under various irradiation doses condition. A dose dependent increase in UV absorbance and development of fluorescence was observed. Free amino groups content showed that up to 5â¯kGy irradiation could accelerate the glycation reaction between OVA and glucose, while there were inapparent differences among the other various doses. Intrinsic, synchronous and 3D fluorescence spectra showed that the glycation reaction caused the dynamic quenching of OVA and changed the polarity of the hydrophobic microenvironment (Trp and Tyr residues) of OVA with unfolding conformation change.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Innovative Food Science & Emerging Technologies - Volume 47, June 2018, Pages 286-291
Journal: Innovative Food Science & Emerging Technologies - Volume 47, June 2018, Pages 286-291
نویسندگان
Guang-xian Liu, Jun Liu, Zong-cai Tu, Xiao-mei Sha, Hui Wang, Zhen-xing Wang,