کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8416376 | 1545569 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimization of germination, callus induction, and cell suspension culture of African locust beans Parkia biglobosa (Jacq.) Benth
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The present study was carried out to determine the best pre-sowing treatments that can enhance the germination and seedling growth of Parkia biglobosa (Jacq.) Also, to establish and long-term maintenance of calli and cell suspension cultures . The result of various pre-sowing treatments showed that seeds soaked in concentrated H2SO4 treatment appeared the highest germination percentage, higher value of plant height, number of leaves, number of branches and stem girth. The MS medium containing 1mg/l 2, 4-D was the best for callus induction of stem explants. The addition of 50Â mg /l citric acid to the MS medium was effective for reducing browning of callus than other treatments. However, the viability percent recorded the maximum (87.76%) on the 9th day while the concentration of viable cells per ml reached the higher record (137.5 viable cell/ml) at the 12th and cell viability remains (â 68.39%) throughout 18 days of culture
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Genetic Engineering and Biotechnology - Volume 16, Issue 1, June 2018, Pages 191-201
Journal: Journal of Genetic Engineering and Biotechnology - Volume 16, Issue 1, June 2018, Pages 191-201
نویسندگان
Mohamed S. Abbas, Hattem M. El-Shabrawi, Amira Sh. Soliman, Mai A. Selim,