کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8464134 | 1549438 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cat preantral follicle survival after prolonged cooled storage followed by vitrification
ترجمه فارسی عنوان
زنده ماندن فولیکول پیش دبرسانه پس از ذخیره سازی طولانی مدت خنک و سپس شیشه سازی
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کلمات کلیدی
VS2CEUAIUCNWS2DPBScFNPBSTem - این استSER - برای بودنoocyte - تخمک گذاریNeutral red - خنثی قرمزRoom temperature - دمای اتاقgranulosa cells - سلولهای گرانولوزاsmooth endoplasmic reticulum - صاف اندوپلاسمی صافNuclear membrane - غشای هسته ایDulbecco's phosphate-buffered saline - فسفات باسیل نمک DulbeccoPhosphate buffered saline - فسفات بافر شورpreantral follicles - فولیکولهای پیش آگهیZona pellucida - منطقه کوچکTransmission electron microscopy - میکروسکوپ الکترونی عبوریPAF - نیروی هوایی پاکستانNucleus - هسته
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
The aim of this study was to investigate the impact of prolonged storage at 4â¯Â°C on survival of cat preantral follicles (PAFs) pre- and post-vitrification. Ovaries were obtained from 12 queens and transported at 4â¯ÂºC within 2-6â¯h. Parts of the ovaries were stored for an additional 24â¯h or 72â¯h. The ovarian cortex was dissected, analyzed for viability (neutral red - NR) and morphology (histology - HE and ultrastructural analysis by TEM) and vitrified. We used 2â¯mm biopsy punches to obtain equal size pieces as the experimental units. After NR assessment, each sample was fixed and embedded in paraffin for HE staining to determine the number of morphologically intact follicles. Another 2â¯mm piece of ovary was subjected to TEM. NR viability assessment and HE results showed a similar tendency with PAF survival postvitrification even after prolonged cooling at 24â¯h and 72â¯h. With TEM, integrity of mitochondria, plasma and basal membranes as well as the presence of pre-granulose cells of PAFs were documented postvitrification for the control group and 24â¯h prolonged storage group, but not after 72â¯h storage. Our results showed that cat PAFs can survive prolonged storage followed by vitrification. The described set of techniques are applicable towards creating a gamete bank for endangered feline species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 81, April 2018, Pages 94-100
Journal: Cryobiology - Volume 81, April 2018, Pages 94-100
نویسندگان
Jorge Luis Araújo Martins, Maria Denise Lopes, Fabiana Ferreira de Souza, Fabio Sossai Possebon, Gudrun Wibbelt, Katarina Jewgenow,