کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8471003 | 1550034 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An apparent increase in meiotic silencing strength in crosses involving inbred Neurospora crassa strains
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
Meiotic silencing by unpaired DNA is a presumed RNAi-mediated elimination of the transcripts of any gene that is not properly paired with a homolog in meiosis. Wild-isolated strains of Neurospora crassa can be classified into three types based on the apparent strength of meiotic silencing of the bml (β-tubulin) and mei-3 genes in crosses of the wild strains with the tester strains ::Bmlr and ::mei-3. Crosses with “OR” and “Sad” type wild-isolates, respectively, did or did not silence both the genes, whereas crosses with the “Esm” type silenced bml, but not mei-3+. Presumably, bml is more sensitive to silencing than mei-3, and silencing is strongest in crosses with the OR type, weakest (or non-evident) in crosses with the Sad type, and of intermediate strength in crosses with Esm type. Now, while constructing recombinant inbred lines from the Sad type wild strains Bichpuri-1 a and Spurger-3 A, we found that although crosses of Bichpuri-1 a and Spurger-3 A, and of most of their f1 progeny, with the ::Bmlr and ::mei-3 testers were consistently and reproducibly Sad type, those of later generation strains of each line could show Sad, Esm, or a variably expressive Sad/Esm type. Since any genotype found in a later generation is, in principle, obtainable in the f1, the observed transition from an apparently stable Sad phenotype to an apparently unstable Sad/Esm phenotype appears not to be due to genotype differences between the generations. Therefore, the Sad versus Esm difference may have a genotype-independent basis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 56, July 2013, Pages 158-162
Journal: Fungal Genetics and Biology - Volume 56, July 2013, Pages 158-162
نویسندگان
T. Nagasowjanya, Kranthi B. Raj, K. Sreethi Reddy, Durgadas P. Kasbekar,