کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8493260 | 1552797 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Does relaxation as a sedative therapy previous to pearl production affect antioxidant response and cause oxidative damage in the winged pearl oyster Pteria sterna?
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Relaxation as a sedative therapy before nucleus seeding is assumed to be stressful to some marine animals during its initial phase, before acting at the cellular level. The antioxidant activity and possible oxidative damage to soft tissues was evaluated in the winged pearl oyster Pteria sterna exposed to four relaxants, at two concentrations each. The relaxants were: propylene phenoxetol (1.5 and 2.5â¯mLâ¯Lâ1), benzocaine (0.15 and 0.25â¯gâ¯Lâ1), magnesium chloride (15 and 30â¯gâ¯Lâ1), and eugenol (0.25 and 0.5â¯mLâ¯Lâ1). The enzymatic activity (superoxide dismutase, catalase, glutathione peroxidase) and oxidative damage (TBARS, lipofuscins) were measured at 30, 60, and 120â¯min after immersion in each relaxant, and at 65â¯h representing the recovery period (filtered seawater with no relaxant). Propylene phenoxetol (2.5â¯mLâ¯Lâ1) and benzocaine (0.25â¯gâ¯Lâ1) induced >80% relaxation within the first 45â¯min. Variations in the activity of superoxide dismutase activity were not significant between treatments in mantle and gills, but increased in muscle at 65â¯h in oysters exposed to 1.5â¯mLâ¯Lâ1 propylene phenoxetol and 15 and 30â¯gâ¯Lâ1 magnesium chloride. Control oysters significantly increased catalase activity in adductor muscle and gills (30-60â¯min), but the activity of glutathione peroxidase did not significant vary in muscle and mantle, and only decreased in gills at 65â¯h exposure to 2.5â¯mLâ¯Lâ1 propylene phenoxetol. Higher TBARS concentrations, as indicative of oxidative damage, only occurred at 30â¯min in muscle and gills in oysters exposed to 30â¯gâ¯Lâ1 magnesium chloride and 0.25â¯gâ¯Lâ1 benzocaine. Relaxing with benzocaine caused a significant accumulation of lipofuscins in gills at 120â¯min, again as possible indicator of oxidative stress; lowest lipofuscins values occurred with 1.5â¯mLâ¯Lâ1 propylene phenoxetol. These results confirm that propylene phenoxetol causes minimal interaction with antioxidant enzymes and provoke little oxidative damage to soft tissues in P. sterna.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 491, 1 April 2018, Pages 295-301
Journal: Aquaculture - Volume 491, 1 April 2018, Pages 295-301
نویسندگان
Andrés Granados-Amores, Pedro E. Saucedo, José M. Mazón-Suástegui, Carmen RodrÃguez-Jaramillo, Héctor Acosta-Salmón, Tania Zenteno-Savin, Ángel I. Campa-Córdova,