کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8526362 | 1557945 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effect of clinically relevant doses of immunosuppressive drugs on human mesenchymal stem cells
ترجمه فارسی عنوان
اثر دوزهای بالینی مرتبط با داروهای ضد سرطان بر سلول های بنیادی مزانشیمی انسانی
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کلمات کلیدی
IDOMSCsmTORCOX-2SDF-1MCP-1MMFPBMCsTGFPhytohemagglutininHGFIMPDHinosine monophosphate dehydrogenaseRAPAPREDTSG-6Dulbeccós modified Eaglés mediumPD-L1DEXDMEMPBSPHADMSO - DMSOCSA - ایالات مؤتلفهٔ آمریکاinterferon - اینترفرونIFN - اینترفرون هاinterleukin - اینترلوکینindoleamine 2,3-dioxygenase - ایندولامین 2،3-دیوکسژیگنازAdipose tissue - بافت چربیtransforming growth factor - تبدیل فاکتور رشدEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاImmunomodulation - تنظیم ایمنی Immunosuppressive drug - داروهای ضد فشارخونDexamethasone - دگزامتازونDimethyl sulfoxide - دیمتیل سولفواکسیدRapamycin - راپامایسینMesenchymal stem cell - سلول های بنیادی مزانشیمیMesenchymal stem cells - سلول های بنیادی مزانشیمیperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیCytokine - سیتوکینCyclosporine A - سیکلوسپورینACyclooxygenase-2 - سیکلوکوکسیژناز2Hepatocyte growth factor - عامل رشد هپاتوسیتStromal-derived factor 1 - عامل مشتق استروما 1Fas Ligand - فاس لیگاندFasL - فاسدGrowth factor - فاکتور رشدVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Phosphate buffered saline - فسفات بافر شورmycophenolate mofetil - مایکوفنولات موفتیلprogrammed death-ligand 1 - مرگ برنامهریزی لیگاند 1bone marrow - مغز استخوانMechanistic target of rapamycin - هدف مکانیکی رپامایسینPlatelet lysate - ورید لازاتmonocyte chemoattractant protein 1 - پروتئین cheoattractant monocyte 1Prednisone - پیشینسونChemokine - کموکاین یا کموکین
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
تومور شناسی
چکیده انگلیسی
Immunosuppressive drugs are used to suppress graft rejection after transplantation and for the treatment of various diseases. The main limitations of their use in clinical settings are severe side effects, therefore alternative approaches are desirable. In this respect, mesenchymal stem cells (MSCs) possess a regenerative and immunomodulatory capacity that has generated considerable interest for their use in cell-based therapy. Currently, MSCs are tested in many clinical trials, including the treatment of diseases which require simultaneous immunosuppressive treatment. Since the molecular targets of immunosuppressive drugs are also present in MSCs, we investigated whether immunosuppressive drugs interact with the activity of MSCs. Human MSCs isolated from the bone marrow (BM) or adipose tissue (AT) were cultured in the presence of clinical doses of five widely used immunosuppressive drugs (cyclosporine A, mycophenolate mofetil, rapamycin, prednisone and dexamethasone), and the influence of these drugs on several factors related to the immunosuppressive properties of MSCs, including the expression of immunomodulatory enzymes, various growth factors, cytokines, chemokines, adhesion molecules and proapoptotic ligands, was assessed. Glucocorticoids, especially dexamethasone, showed the most prominent effects on both types of MSCs and suppressed the expression of the majority of the factors that were tested. A significant increase of hepatocyte growth factor production in AT-MSCs and of indoleamine 2,3-dioxygenase expression in both types of MSCs were the only exceptions. In conclusion, clinically relevant doses of inhibitors of calcineurin, mTOR and IMPDH and glucocorticoids interfere with MSC functions, but do not restrain their immunosuppressive properties. These findings should be taken into account before preparing immunosuppressive strategies combining the use of immunosuppressive drugs and MSCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 97, January 2018, Pages 402-411
Journal: Biomedicine & Pharmacotherapy - Volume 97, January 2018, Pages 402-411
نویسندگان
Eliska Javorkova, Julie Vackova, Michaela Hajkova, Barbora Hermankova, Alena Zajicova, Vladimir Holan, Magdalena Krulova,