کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8545681 | 1561681 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Titanium dioxide nanoparticles induce mouse hippocampal neuron apoptosis via oxidative stress- and calcium imbalance-mediated endoplasmic reticulum stress
ترجمه فارسی عنوان
نانوذرات دی اکسید تیتانیوم باعث آپوپتوز عضله هیپوکامپ ماوس از طریق استرس ایزوله و تنش ریشه ای اندوپلاسمی ناشی از عدم تعادل کلسیم
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کلمات کلیدی
DLSDAPIDCFH-DAERSN-acetyl-l-cysteineIRE1ATF6NACDcfGRP78FBSDMEM3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideAO/EB - AO / EBBSA - BSACa2+ - Ca2 +Dulbecco’s modified Eagle medium - Modified Eagle اصلاح شده DulbeccoMTT - MTTacridine orange/ethidium bromide - آکاردین نارنجی / اتیدیم برومیدEndoplasmic reticulum stress - استرس شبکه آندوپلاسمیBBB - سد خونی مغزیfetal bovine serum - سرم جنین گاوbovine serum - سرم گاوendoplasmic reticulum - شبکه آندوپلاسمی activating transcription factor 6 - فعال کردن عامل رونویسی 6Blood-brain barrier - مانع خون مغزیDynamic Light Scattering - پراکندگی نور دینامیکیPERK - پرکCalcium - کلسیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
The purpose of this study was to explore the potential neurotoxicity and the underlying mechanism of titanium dioxide nanoparticles (TiO2-NPs) to mouse hippocampal neuron HT22 cells. We found that TiO2-NPs had concentration-dependent and time-dependent cytotoxicities to HT22 cells by the MTT assay. Propidium iodide (PI) staining with FACScan flow cytometry proved that TiO2-NPs dose-dependently increased the apoptosis rate in HT22 cells, and the apoptotic features were observed by Hochest 33258 and AO/EB staining. The levels of calcium (Ca2+) and reactive oxygen species (ROS) were significantly increased in TiO2-NPs-treated cells. Further studies by western blot and real-time QPCR proved that the protein and mRNA levels of GRP78, IRE-1α, ATF6, CHOP and caspase-12 were up-regulated after TiO2-NPs treatment, which indicates that TiO2-NPs-induced cytotoxicity is related to endoplasmic reticulum stress (ERS). Apoptosis-related protein cleaved caspase-3 and pro-apoptotic protein Bax expression levels were up-regulated, and the anti-apoptotic protein Bcl-2 expression level was down-regulated in TiO2-NPs-treated cells. The antioxidant N-acetyl-L-cysteine (NAC) can significantly reduce TiO2-NPs-induced ERS characterized by the down-regulation of GRP78 and cleaved caspase-12 levels, which indicates that oxidative stress is participated in TiO2-NPs-induced ERS. Our study suggests that TiO2-NPs-induced apoptosis in HT22 cells is through oxidative stress- and calcium imbalance-mediated ERS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 63, October 2018, Pages 6-15
Journal: Environmental Toxicology and Pharmacology - Volume 63, October 2018, Pages 6-15
نویسندگان
Qiong He, Xuejiao Zhou, Yang Liu, Wenfeng Gou, Jiahui Cui, Zengqiang Li, Yingliang Wu, Daiying Zuo,