کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8546409 | 1561725 | 2018 | 42 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The mechanism of DNA damage induced by Roundup 360 PLUS, glyphosate and AMPA in human peripheral blood mononuclear cells - genotoxic risk assessement
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
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چکیده انگلیسی
Glyphosate is the most heavily applied among pesticides in the world, and thus human exposure to this substance continues to increase. WHO changed classification of glyphosate to probably cancerogenic to humans, thus there is urgent need to assess in detail genotoxic mechanism of its action. We have assessed the effect of glyphosate, its formulation (Roundup 360 PLUS) and its main metabolite (aminomethylphosphonic acid, AMPA) in the concentration range from 1 to 1000â¯Î¼M on DNA damage in human peripheral blood mononuclear cells (PBMCs). The cells were incubated for 24â¯h. The compounds studied and formulation induced DNA single and double strand-breaks and caused purines and pyrimidines oxidation. None of compounds examined was capable of creating adducts with DNA, while those substances increased ROS (including
- OH) level in PBMCs. Roundup 360 PLUS caused damage to DNA even at 5â¯Î¼M, while glyphosate and particularly AMPA induced DNA lesions from the concentration of 250â¯Î¼M and 500â¯Î¼M, respectively. DNA damage induced by glyphosate and its derivatives increased in order: AMPA, glyphosate, Roundup 360 PLUS. We may conclude that observed changes were not associated with direct interaction of xenobiotics studied with DNA, but the most probably they occurred through ROS-mediated effects.
- OH) level in PBMCs. Roundup 360 PLUS caused damage to DNA even at 5â¯Î¼M, while glyphosate and particularly AMPA induced DNA lesions from the concentration of 250â¯Î¼M and 500â¯Î¼M, respectively. DNA damage induced by glyphosate and its derivatives increased in order: AMPA, glyphosate, Roundup 360 PLUS. We may conclude that observed changes were not associated with direct interaction of xenobiotics studied with DNA, but the most probably they occurred through ROS-mediated effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 120, October 2018, Pages 510-522
Journal: Food and Chemical Toxicology - Volume 120, October 2018, Pages 510-522
نویسندگان
Ewelina Woźniak, Paulina SiciÅska, Jaromir MichaÅowicz, Katarzyna Woźniak, Edyta Reszka, BogumiÅa Huras, Jerzy Zakrzewski, Bożena Bukowska,