کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8553200 | 1562580 | 2018 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In utero and lactational exposure of DEHP increases the susceptibility of prostate carcinogenesis in male offspring through PSCA hypomethylation
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کلمات کلیدی
DEHPESR2GSTP1PSQPtgs2PSCARASSF1ANKX3.1rps16PNDglutathione S-transferase Pi 1PSAEstrogen receptor 2prostatic intraepithelial neoplasia - neoplasia intraepithelial پروستاتPCA - PCAS.D. - SD.Prostate specific antigen - آنتی ژن اختصاصی پروستاتProstate stem cell antigen - آنتی ژن سلول بنیادی پروستاتstandard deviation - انحراف معیارANOVA - تحلیل واریانس Analysis of varianceone-way analysis of variance - تحلیل واریانس یک راههdi(2-ethylhexyl) phthalate - دی (2-اتیل هگزیل) فتالاتgestational day - روز بارداریpostnatal day - روز پس از زایمانProstate carcinogenesis - سرطان زایی پروستاتProstate cancer - سرطان پروستاتDNA methylation - متیلاسیون DNAprostaglandin-endoperoxide synthase 2 - پروستاگلاندین اندپورکسید سنتاز 2Pyrosequencing - پیروسکوئنسینگ PIN - پین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
As an ubiquitous environmental endocrine disruptor, di(2-ethylhexyl) phthalate (DEHP) has been shown to interfere with the development of reproductive organs and induce pathological changes in prostate. Our previous finding showed that in utero and lactational (IUL) DEHP exposure could disrupt the balance of testosterone and estrogen and increase the susceptibility of prostate carcinogenesis. The purpose of this study is to investigate whether the early-life specific epigenetic modifications could mediate the effect of DEHP exposure on prostate carcinogenesis in rodents, for epigenetic modifications play important roles in regulating prostate carcinogenesis. The pregnant rats were treated with corn oil (negative control) or DEHP at 0.01, 0.1 and 1â¯mg/kg BW/day from GD7 to PND21. On PND21, the expression and DNA methylation change of six prostate carcinogenesis-related genes (ESR2/GSTP1/NKX3.1/PSCA/PTGS2/Rassf1a) were assessed through SYBR-Green real-time PCR combined with pyrosequencing assay in F1 male offspring. On PND196, the relationship b(STP1, PSCA and PTGS2 in a dose-dependent manner, which were positively correlated with PIN scores, Gleason scores, serum PSA concentrations and negatively correlated with prostate/body weight ratio on PND196. Meanwhile, 1â¯mg/kg BW/day DEHP markedly reduced DNA methylation level of PSCA in all studied CpG sites. Significant inverse correlations between methylation levels of the promoter CpG site and PSCA mRNA expression were observed. These results indicated that transcriptional changes of GSTP1, PSCA and PTGS2 induced by DEHP exposure might be contribute to the increasing susceptibility of prostate carcinogenesis in late life. Moreover, hypomethylation of PSCA could mediate the effect of DEHP on prostate carcinogenesis in rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 292, August 2018, Pages 78-84
Journal: Toxicology Letters - Volume 292, August 2018, Pages 78-84
نویسندگان
Bin Xia, Yong Wang, Xiu Wang, Jianhui Wu, Qi Song, Zuyue Sun, Yunhui Zhang,