One-pot construction of mediatorless bi-enzymatic glucose biosensor based on organic–inorganic hybrid

A new methodology for the fabrication of bienzymatic amperometric glucose biosensor based on the use of an organic–inorganic hybrid is presented. The fabrication involves a self-assembly directed one-pot electrochemical process. Bi-enzymes, horseradish peroxidase (HRP) and glucose oxidase (GOx) are immobilized into the porous and electroactive silica–polyaniline hybrid composite through electrochemical polymerization of N[3-(trimethoxysilyl)propyl]aniline in the presence of enzymes. The modified electrode is designated as PTMSPA/HRP-GOx. The direct electron transfer of HRP is achieved at the modified electrode. Also, the electrode exhibits excellent bio-electro-catalytic activity for the reduction of hydrogen peroxide. The response current at PTMSPA/HRP-GOx modified electrode revealed a good linear relationship with concentration of glucose range between 1 and 20 mM with a response time of 7 s. Thus, the modified electrode shows the combined advantages of polyaniline and silica networks through synergistic influence from the individual components. The PTMSPA assembly has shown the potential for a third generation amperometric biosensor.