کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8791877 | 1602546 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular and functional characterization of circulating extracellular vesicles from diabetic patients with and without retinopathy and healthy subjects
ترجمه فارسی عنوان
خصوصیات مولکولی و عملکردی حاملهای خارج سلول گردش خون از بیماران دیابتی با و بدون رتینوپاتی و افراد سالم
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کلمات کلیدی
ECMhypoxia-inducible factor 1 αmiR-150-5pPericytesHIF-1αCTRHMECMSCHRPmiR, microRNA - miR، microRNAAngiogenesis - آنژیوژنزDiabetes - بیماری قندExtracellular vesicles - حامل های خارج سلولیKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوdiabetic retinopathy - رتینوپاتی دیابتیEndothelial cell - سلول های اندوتلیالMesenchymal stem cells - سلول های بنیادی مزانشیمیhuman microvascular endothelial cells - سلولهای اندوتلیال میکرواسکولار انسانVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Phosphatidyl-serine - فسفاتیدیل سریینExtracellular matrix - ماتریکس خارج سلولیMiRNA - میکروRNA، ریزآرانای، miRNA
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی و میکروب شناسی (عمومی)
چکیده انگلیسی
Diabetic retinopathy is a sight-threatening complication of diabetes, characterized by loss of retinal pericytes and abnormal angiogenesis. We previously demonstrated that extracellular vesicles (EVs) derived from mesenchymal stem cells cultured in diabetic-like conditions are able to enter the pericytes, causing their detachment and migration, and stimulating angiogenesis in vitro. The purpose of this work was the molecular and functional characterization of EVs derived from diabetic subjects with or without diabetic retinopathy, compared with healthy controls. Characterization of EVs extracted from serum/plasma of diabetic patients with or without retinopathy, and healthy controls, was performed by FACS and microarray analysis of microRNA (miRNA) content. Relevant miRNA expression was validated through qRT-PCR. EV influence on pericyte detachment, angiogenesis and permeability of the blood-retinal barrier was also investigated. Diabetic subjects had a 2.5 fold higher EV concentration than controls, while expression of surface molecules was unchanged. Microarray analysis revealed 11 differentially expressed miRNAs. Three of them (miR-150-5p, miR-21-3p and miR-30b-5p) were confirmed by qRT-PCR. Plasma EVs from subjects with diabetic retinopathy induced pericyte detachment and pericyte/endothelial cell migration, increased the permeability of pericyte/endothelial cell bilayers and the formation of vessel-like structures, when compared with EVs from controls. In conclusion, circulating EVs show differences between diabetic patients and healthy subjects. EVs extracted from plasma of diabetic retinopathy patients are able to induce features of retinopathy in in vitro models of retinal microvasculature. Our data suggest a role for miR-150-5p, miR-21-3p and miR-30b-5p as potential biomarkers of the onset of diabetic retinopathy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 176, November 2018, Pages 69-77
Journal: Experimental Eye Research - Volume 176, November 2018, Pages 69-77
نویسندگان
Aurora Mazzeo, Elena Beltramo, Tatiana Lopatina, Chiara Gai, Marina Trento, Massimo Porta,