کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8959794 1646357 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A preliminary assessment of the effect of PreCR™ DNA repair treatment on mixture ratios in two person mixtures
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A preliminary assessment of the effect of PreCR™ DNA repair treatment on mixture ratios in two person mixtures
چکیده انگلیسی
In this study, DNA extracted from known buccal samples was combined into two component mixture samples. These were subjected to UV exposure prior to their amplification with the Promega PowerPlex® 16HS amplification kit, and subsequent capillary electrophoresis on the ABI 3130xl instrument. Damaged samples were subjected to enzymatic repair treatment and retested to assess the amount of repair. Data showed that there is fidelity associated with the application with profile concordance after its use, and a corresponding increase in the amount of recovered alleles post damage. Results also showed changes in the stochastic relationship between mixture components that appear to be induced by the repair process itself. The mixture ratios of DNA samples were altered from an approximate original 1:3 ratio, to a ratio of 1:2 or greater. This variation can have a significant effect regarding the ability to reliably de-convolute DNA mixtures that have been subjected to the repair process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science & Justice - Volume 58, Issue 5, September 2018, Pages 308-314
نویسندگان
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