کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8961158 1646472 2018 21 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Probing selectivity of mixed-mode reversed-phase/weak-anion-exchange liquid chromatography to advance method development
ترجمه فارسی عنوان
انتخاب روش انتخابی کروماتوگرافی مایع انتقالی فازی متناوب / حالت ضعیف آنیون برای پیشبرد روش توسعه
کلمات کلیدی
مشخصه ستون، مکانیزم نگهداری، توسعه روش، مدل سازی زمان نگهداری،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The current study comprises a systematic investigation to assess retention properties and selectivity of a mixed-mode reversed-phase/weak-anion-exchange (RP/WAX) stationary phase to aid method development. Retention was investigated for different compound classes which vary in hydrophobicity, van der Waals surface area, and charge as function of organic content, pH, and ionic strength of the mobile phase. The linear-solvent-strength model was successfully applied for aromatic hydrocarbons to obtain retention-time predictions based on log P values and van der Waals surface area values. For phenols, predictions were based on log P values and data from a single scouting run performed in isocratic mode to estimate the S parameter; the deviations between experimental and predicted retention times were smaller than 6%. To describe the mixed-mode (RP/WAX) retention behavior of singly and doubly negatively-charged aromatic acids, a novel model combining the linear-solvent-strength and the empirical stoichiometric-displacement-net-charge models is proposed and validated. Using combinations of three scouting runs that are not linearly dependent, the maximum prediction error was 11% and changes in selectivity were correctly forecasted when altering the mobile-phase composition, i.e., either organic modifier content or ionic strength. When using nine scouting runs in combination with a least-squares regression approach to determine the model parameters, the maximum prediction error was 6%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1570, 5 October 2018, Pages 75-81
نویسندگان
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