کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8969777 | 1103294 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An integral approach towards a practical application for a plant-made monoclonal antibody in vaccine purification
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The use of transgenic plants for the production of pharmaceutical compounds has received increasing attention in the last few years. However, many technological and regulatory issues regarding the practical exploitation of this alternative system of production remain to be solved; a situation that explains the lack of commercial products derived from such a system. This paper reports the expression in transgenic plants and cells of a single-chain antibody variable-region fragment (scFv) and a mouse monoclonal antibody to the hepatitis B virus surface antigen (HBsAg). The large-scale purification of the scFv from plants and its use for immunopurification of HBsAg are also described, together with elements concerning regulatory issues and technologies for compliance with good manufacturing and agricultural practices.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 23, Issue 15, 7 March 2005, Pages 1833-1837
Journal: Vaccine - Volume 23, Issue 15, 7 March 2005, Pages 1833-1837
نویسندگان
Merardo Pujol, Nadia I. RamÃrez, Marta Ayala, Jorge V. Gavilondo, Rodolfo Valdés, Meilyn RodrÃguez, José Brito, Sigifredo Padilla, Leonardo Gómez, Biunaiky Reyes, Rudy Peral, Marlene Pérez, José L. Marcelo, Lorely Milá, Rafael F. Sánchez,