کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9009798 | 1124086 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of Bambusae concretio Salicea (Chunchukhwang) on amyloid β-induced cell toxicity and antioxidative enzymes in cultured rat neuronal astrocytes
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کلمات کلیدی
APPNBTCDNBGPXAβGSTGSHNADPHTEPTBANaN3XODDETAPAC1,1,3,3-tetraethoxypropaneFBSDMEMPBS1-chloro-2,4-dinitrobenzene - 1-کلرو-2،4-دینیتروبنزن3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideDMSO - DMSODulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoMTT - MTTAstrocyte - آستروسیتamyloid-β - آمیلوئید βAntioxidative enzyme - آنزیم آنتیاکسیدانیEDTA - اتیلن دی آمین تترا استیک اسید ethylenediamine tetraacetic acid - اسید اتیلنیدین تتراستیکdiethylene triamine pentaacetic acid - اسید پنتاتیک دی اتیل تریامینAlzheimer's disease - بیماری آلزایمرnitro blue tetrazolium - تترازولیم نیترو آبیdimethyl sulphoxide - دی متیل سولفوکسیدBCs - روند BCsSOD - سدsodium azide - سدیم آیزیدfetal bovine serum - سرم جنین گاوSuperoxide dismutase - سوکسوکس دیسموتازLDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریnicotinamide adenine dinucleotide phosphate - نیکوتین آمید adenine dinucleotide phosphateamyloid precursor protein - پروتئین پیش ماده آمیلوئیreduced glutathione - کاهش گلوتاتیونglutathione-S-transferase - گلوتاتیون S-ترانسفرازglutathione peroxidase - گلوتاتیون پراکسیداز
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effects of Bambusae concretio Salicea (Chunchukhwang) on amyloid β-induced cell toxicity and antioxidative enzymes in cultured rat neuronal astrocytes Effects of Bambusae concretio Salicea (Chunchukhwang) on amyloid β-induced cell toxicity and antioxidative enzymes in cultured rat neuronal astrocytes](/preview/png/9009798.png)
چکیده انگلیسی
Bambusae concretio Salicea (BCS; plant family name: Phyllostachys bambusoides Siebold et Zuccarinii) is a medicinal plant used in Korea for the treatment of various symptoms accompanying hypertension and cerebrovascular disorders. Previously, it was shown that BCS is an effective protectant against oxidative glutamate toxicity in the murine neuroblastoma cells and human neuroblastoma cells. Treatment with BCS increased the secretion of the non-amyloidogenic amyloid precursor protein fragment, and decreased the secretion of amyloid-β (Aβ) peptides from neuronal cells [Jeong, J.C., Seo, Y.J., Kim, H.M., Lee, Y.C., Kim, C.H., 2003. Inhibitory effects of Bombusae concretio Salicea on neuronal secretion of Alzheimer's β-amyloid peptides, a neuro-degenerative peptide. Neurochemical Research 28, 1785-1792.]. To further examine the pharmacological activity of BCS, we studied the protective effect of the water extracts on Aβ25-35 peptide-induced neuronal death by microscopic observation and lactate dehydrogenase (LDH) assay, and action on antioxidative enzymes using cultured astrocyte cells. Ten μM Aβ25-35-induced cell death was protected by the application of water extract of BCS in a dose-dependent manner, and concentrations of 1-10 μg/ml had a significant effect compared to exposure to Aβ25-35 only. When antioxidative enzyme activities such as catalase, superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were assayed after Aβ25-35 treatment, the enzymes were decreased in a similar fashion. However, those activities were enhanced by BCS treatment and this may have resulted from the potentiation of antioxidative ability by BCS. The ability of BCS to reduce cellular cytotoxicity induced by 10 μM Aβ25-35 suggests that BCS may be a protective agent for free radical generating compounds such as Aβ25-35, and that Aβ25-35 is not only a potent lipid peroxide inducer, but also causes changes in antioxidative enzymes. From the results, it was concluded that BCS has a protective effect on Aβ-induced neuronal death in cultured astrocyte cells through the inhibition of lipid peroxidation and protection of antioxidative enzymes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 98, Issue 3, 26 April 2005, Pages 259-266
Journal: Journal of Ethnopharmacology - Volume 98, Issue 3, 26 April 2005, Pages 259-266
نویسندگان
Ji-Cheon Jeong, Cheol-Ho Yoon, Woo-Hun Lee, Kwan-Kyu Park, Young-Chae Chang, Yung Hyun Choi, Cheorl-Ho Kim,