کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9110778 | 1155365 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cytokine interactions in mesenchymal stem cells from cord blood
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کلمات کلیدی
pulmonary and activation-regulated chemokineMCPMIPOSMENA-78granulocyte chemotactic proteinFGFIGFBPPARCGCPPIGFTGFoncostatin M - اوکتواستاتین مinterleukin-6 - اینترلوکین ۶Interleukin-1β - اینترلوکین-1βtissue inhibitors of matrix metalloproteinases - بازدارنده های بافت متالوپروتئیناز ماتریکسtransforming growth factor - تبدیل فاکتور رشدCord blood - خون بند نافgrowth-related oncogene - رشد انکوژنTIMP - زمانMesenchymal stem cell - سلول های بنیادی مزانشیمیCytokine - سیتوکینMIF - شهرMacrophage migration inhibitory factor - عامل مهارکننده مهاجرت ماکروفاژfibroblast growth factor - فاکتور رشد فیبروبلاستmonocyte chemotactic protein - پروتئین chemotactic monocyteinsulin-like growth factor binding protein - پروتئین اتصال دهنده به عامل انسولین مانندmacrophage inflammatory protein - پروتئین التهابی ماکروفاژinterferon-inducible protein - پروتئین القا کننده اینترفرونGro - گور
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We used cytokine protein array to analyze the expression of cytokines from human cord blood-derived mesenchymal stem cells (CB-MSCs). Several cytokines, interleukins (IL), and growth factors, including ENA-78, GM-CSF, GRO, IL-1β, IL-6, IL-8, MCP-1, OSM, VEGF, FGF-4, FGF-7, FGF-9, GCP-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IP-10, LIF, MIF, MIP-3α, osteoprotegerin, PARC, PIGF, TGF-β2, TGF-β3, TIMP-1, as well as TIMP-2, were secreted by CB-MSCs, while IL-4, IL-5, IL-7, IL-13, TGF-β1, TNF-α, and TNF-β were not expressed under normal growth conditions. IL-6, IL-8, TIMP-1, and TIMP-2 were the most abundant interleukins expressed by CB-MSCs. A set of growth factors were selected to evaluate their stimulatory effects on the IL6 secretion for CB-MSCs. IL-1β was the most important factor inducing CB-MSC to secret IL-6. The mechanism by which IL-1β promoted IL-6 expression in CB-MSCs was studied. By using various inhibitors of signal transduction, we found that activation of p38 mitogen-activated protein kinases (MAPK) and MAPK kinase (MEK) is essential in the IL-1β stimulated signaling cascade which leads to the increase in IL-6 synthesis. Additionally, continuous supplement of IL-1β in the CB-MSCs culture will facilitate adipogenic maturation of CB-MSCs as evidenced by the presence of oil drops in the CB-MSCs and secretion of leptin, a molecule marker of adipocytes. These results strongly suggest that cytokine induction and signal transduction are important for the differentiation of CB-MSCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 32, Issue 6, 21 December 2005, Pages 270-279
Journal: Cytokine - Volume 32, Issue 6, 21 December 2005, Pages 270-279
نویسندگان
Chi-Hsien Liu, Shiaw-Min Hwang,