کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9121284 | 1159183 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In situ analysis of native microbial communities in complex samples with high particulate loads
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: In situ analysis of native microbial communities in complex samples with high particulate loads In situ analysis of native microbial communities in complex samples with high particulate loads](/preview/png/9121284.png)
چکیده انگلیسی
In the present study a procedure combining a cell extraction method and Fluorescence In Situ Hybridization (FISH) for molecular monitoring and quantification of bacteria in soil and aquifer samples is presented. FISH was applied to bacterial cells extracted from the matrix by density gradient centrifugation. This separation method was applied to soil and aquifer samples and produced high cell recovery of 76.5% ± 4.4 and 78.0% ± 3.2, respectively. FISH, performed on the harvested cells, permitted a perfect visualization and quantification of bacteria. This approach is therefore promising for in situ detection of indigenous bacterial communities in complex samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 253, Issue 1, 1 December 2005, Pages 55-58
Journal: FEMS Microbiology Letters - Volume 253, Issue 1, 1 December 2005, Pages 55-58
نویسندگان
Anna Barra Caracciolo, Paola Grenni, Cinzia Cupo, Simona Rossetti,