کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9126933 | 1569965 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A microarray analysis of developmentally regulated genes during macronuclear differentiation in the stichotrichous ciliate Stylonychia lemnae
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کلمات کلیدی
HmgNuclear differentiationRF-CPCNARRMRecAMDPIESRNA interference - RNA تداخل کنندهSmall interfering RNA - RNA تداخل کوچکRNA recognition motif - RNA تشخیص موتیفRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهsiRNA - siRNAProliferating Cell Nuclear Antigen - آنتیژن هسته ای تکثیر سلولیBasic Local Alignment Search Tool - ابزار جستجوی محلی سازگاری محلیE-value - ارزش EExpectation value - ارزش انتظارGene expression pattern - الگوی بیان ژنBlast - انفجارinternal eliminated sequences - توالی های حذف شده داخلیVegetative cells - سلول های گیاهیciliates - سیلاب هاcDNA microarray - میکروآرایه cDNAhigh mobility group - گروه تحرک بالا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
After sexual reproduction in ciliated protozoa a new macronucleus differentiates from a micronuclear derivative. In the course of macronuclear development dramatic DNA- and chromatin reorganisation processes occur, which include splicing of DNA sequences such as IES (internal eliminated sequences) and transposon-like elements during formation of polytene chromosomes, degradation of the polytene chromosomes and specific elimination of micronuclear-specific DNA, de novo addition of telomeres and specific amplification of DNA sequences. In order to understand the molecular basis of this nuclear differentiation process, analysis of developmentally regulated genes seems to be a necessary prerequisite. We performed a microarray analysis to identify genes differentially expressed during macronuclear differentiation. 467 sequences from cDNA libraries were identified as possible candidates from which 384 sequences were further characterised by sequence analysis. These sequences were identified, if possible, by DNA and protein BLAST analysis. Expression of one of these sequences was silenced by RNAi and a preliminary functional analysis performed. Results presented in this study provide the basis for a functional characterisation of genes differentially expressed during this nuclear differentiation process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 359, 10 October 2005, Pages 81-90
Journal: Gene - Volume 359, 10 October 2005, Pages 81-90
نویسندگان
Adrian G. Paschka, Volker Horejschi, Franziska Jönsson, Antje Lindecke, Gero Weier, Christian Kaltschmidt, Hans J. Lipps,