کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9331486 | 1600725 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lymphocyte phenotyping and NK cell activity analysis in pregnant NOD/SCID mice
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کلمات کلیدی
NODmAbASGM1FSCSSCPolyICSCIDanti-asialo GM1Uterine NK cellMonoclonal antibody - آنتی بادی مونوکلونالReproductive immunology - ایمونولوژی تولید مثلImmunodeficiency diseases - بیماری های ایمنیPlacenta - جفت Animal model - مدل حیوانیnon-obese diabetic mouse - موش دیابتی غیر چاقیpolyinosinic-polycytidylic acid - پلیسی سمی-پلیسییدیدیل اسیدsevere combined immunodeficiency - کمبود شدید مصدوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The purpose of this study was to outline the fertility features of non-obese diabetic (NOD)/LtSz-scid/scid (NOD/SCID [severe combined immunodeficiency] for short) mice, and to evaluate the effects of NK cell subsets on the pregnancy outcomes of the syngeneic NOD/SCID Ã NOD/SCID mating combination. Firstly, lymphocyte phenotyping was performed with flow cytometry to confirm the multiple immunodeficits in NOD/SCID mice. Fertility features were assessed in NOD/SCID Ã NOD/SCID mice and compared with non-immunodeficiency control BALB/c Ã BALB/c mice. Although the presence of NK cell deficit is apparent in NOD/SCID mice, a certain level of remnant NK activity could be observed in these mice. The remnant NK cell activity was stimulated with polyinosinic-polycytidylic acid (polyIC), or inhibited with anti-asialo GM1 (ASGM1) anti-serum, respectively. The effects of these factors on pregnancy outcomes were evaluated after administration. Roughly normal fertility could be observed in NOD/SCID Ã NOD/SCID mice. However, a slightly larger sized litter was observed in polyIC-treated NOD/SCID Ã NOD/SCID mice compared with control NOD/SCID mice. In contrast, embryo resorption was boosted after ASGM1 injection, and correlated subsequently with a smaller litter size. It indicates that the remnant NK cell activity in NOD/SCID mice may be beneficial to feto-maternal tolerance during pregnancy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Reproductive Immunology - Volume 68, Issues 1â2, December 2005, Pages 39-51
Journal: Journal of Reproductive Immunology - Volume 68, Issues 1â2, December 2005, Pages 39-51
نویسندگان
Yi Lin, Yijing Chen, Yaoying Zeng, Tong Wang, Shan Zeng,