کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9439936 1615932 2005 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of T- and C-loop mutations on the Herbaspirillum seropedicae GlnB protein in nitrogen signalling
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Effect of T- and C-loop mutations on the Herbaspirillum seropedicae GlnB protein in nitrogen signalling
چکیده انگلیسی
Proteins of the PII family are found in species of all kingdoms. Although these proteins usually share high identity, their functions are specific to the different organisms. Comparison of structural data from Escherichia coli GlnB and GlnK and Herbaspirillum seropedicae GlnB showed that the T-loop and C-terminus were variable regions. To evaluate the role of these regions in signal transduction by the H. seropedicae GlnB protein, four mutants were constructed: Y51F, G108A/P109a, G108W and Q3R/T5A. The activities of the native and mutated proteins were assayed in an E. coli background constitutively expressing the Klebsiella pneumoniae nifLA operon. The results suggested that the T-loop and C-terminus regions of H. seropedicae GlnB are involved in nitrogen signal transduction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 156, Issues 5–6, June–July 2005, Pages 634-640
نویسندگان
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