کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9678415 | 1454326 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neutron and X-ray scattering studies of cholera toxin interactions with lipid monolayers at the air-liquid interface
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
شیمی کلوئیدی و سطحی
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چکیده انگلیسی
Using neutron/X-ray reflectivity and X-ray grazing incidence diffraction (GID), we have characterized the structure of mixed DPPE:GM1 lipid monolayers before and during the binding of cholera toxin (CTAB5) or its B subunit (CTB5). Structural parameters such as the density and thickness of the lipid layer, extension of the GM1 oligosaccharide headgroup, and orientation and position of the protein upon binding are reported. Both CTAB5 and CTB5 were measured to have â¼50% coverage when bound to the lipid monolayer. X-ray GID experiments show that both the lipid monolayer and the cholera toxin layer are crystalline. The effects of X-ray beam damage have been assessed and the monolayer/toxin structure does not change with time after protein binding has saturated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Colloids and Surfaces B: Biointerfaces - Volume 40, Issues 3â4, 25 February 2005, Pages 159-163
Journal: Colloids and Surfaces B: Biointerfaces - Volume 40, Issues 3â4, 25 February 2005, Pages 159-163
نویسندگان
C.E. Miller, J. Majewski, K. Kjær, M. Weygand, R. Faller, S. Satija, T.L. Kuhl,