کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9748896 | 1493795 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of a new methylated β-cyclodextrin with a low degree of substitution by matrix-assisted laser desorption/ionization mass spectrometry and liquid chromatography using evaporative light scattering detection
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کلمات کلیدی
Methylated β-cyclodextrinsmatrix-assisted laser desorption/ionization time-of-flight mass spectrometry - اسپکترومتر جرمی زمان یخ زدن لیزر جذب / یونیزاسیون ماتریسEvaporative light scattering detection - تشخیص پراکندگی نور تبخیریDegree of substitution - درجه جایگزینیhigh-performance liquid chromatography - کروماتوگرافی مایعی کارا
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A new methylated β-cyclodextrin with a low degree of substitution was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and high-performance liquid chromatography (HPLC) with evaporative light scattering detection. Using α-cyano-4-hydroxycinnamic acid as the matrix and the thin layer method as the deposition procedure, MALDI-TOF-MS revealed that the mixture was composed of CDs bearing from 2 to 8 methyl groups with an average degree of substitution (DS) of 0.7 (i.e. 0.7 methyl groups per glucopyranose unit). Using a Purospher Star RP-18 endcapped column with acetonitrile-water mobile phase in gradient elution mode, HPLC was employed at analytical scale to obtain a chromatographic fingerprint of the crude mixture and at semi-preparative scale to fractionate it. MALDI-TOF-MS of these fractions revealed that the overall retention of the different derivatives, which depicts their polarity, was mainly driven by the DS and increased with the number of methyl groups on the CD moiety.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1083, Issues 1â2, 12 August 2005, Pages 106-112
Journal: Journal of Chromatography A - Volume 1083, Issues 1â2, 12 August 2005, Pages 106-112
نویسندگان
Romain Jacquet, Patrick Favetta, Claire Elfakir, Michel Lafosse,