کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9902271 | 1545797 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An improved methodology to detect human T cell receptor beta variable family gene expression patterns
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کلمات کلیدی
black hole quencherFAMBHQTCrNCBIReal-time PCR - PCR به موقعcycle threshold - آستانه چرخهPrimers - آغازگرهاAllele - آللBasic Local Alignment Search Tool - ابزار جستجوی محلی سازگاری محلیBlast - انفجارSubfamily - زیرشاخهWorld Health Organization - سازمان بهداشت جهانیT cell - سلول تیNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیconstant region - منطقه ثابتvariable region - منطقه متغیرpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازcarboxyfluorescein - کربوکسی فلوئورسینWHO - کهT cell receptor - گیرنده سلول T
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Comprehensive gene expression analysis of the T cell receptor repertoire of an individual can be very useful in evaluating the immune response in a variety of conditions. Antibody-based analysis methods can detect approximately 60% of the human T cell receptor beta variable (TCRBV) proteins, while gene expression analysis, primarily through employment of the polymerase chain reaction (PCR), has had somewhat greater success in the detection of additional TCRBV families. Many of these previous PCR methods, however, have been unable to detect all 91 alleles of the human TCRBV genes. This is primarily due to either deficiencies in the amplification of all of the variable beta families, subfamilies, and alleles, or the prior lack of a systematic classification of the TCR variable family gene segment sequences. We describe here a real-time reverse transcription polymerase chain reaction-based method, which allows efficient automation and integration of amplification, detection, and analysis with sequence-specific detection of all T cell receptor beta variable gene families, subfamilies, and alleles. This method, which in itself contributes significant improvements over existing technologies through its comprehensiveness and efficiency, also functions independently of variables such as sample source and sample processing and has the ability to run on multiple real-time PCR platforms, affording one the implementation of personal preferences.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 302, Issues 1â2, July 2005, Pages 54-67
Journal: Journal of Immunological Methods - Volume 302, Issues 1â2, July 2005, Pages 54-67
نویسندگان
Jamie Leigh Brewer, Solveig Gronning Ericson,