Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; ES cell, embryonic stem cell; CSCs, cancer stem cells; IR, ionizing radiation; RT-PCR, reverse transcription polymerase chain reaction; Cy3, cyanine 3; Cy5, cyanine 5; GO, gene ontology; Gy, gray; qRT-PCR, quantitative real-time RT-PCR; SD, standard devia
مقالات ISI RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس (ترجمه نشده)
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Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; hMPV, human metapneumovirus; RT-PCR, reverse transcription polymerase chain reaction; RTIs, respiratory tract infections; ICU, intensive care unit; F, fusion protein; G, glycoprotein; RNA, ribonucleic acid; nt, nucleotide; bp, base pair; kb, kilobaseRespi
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; HPeV, human parechovirus; EV, human enterovirus; CNS, central nervous system; MRI, magnetic resonance imaging; RT-PCR, reverse transcription polymerase chain reaction; CSF, cerebral spinal fluid; DWI, diffusion-weighted imaging; ICU, intensive care unitPa
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; ELISA, enzyme linked immunosorbent assay; IA, immunoassay; RT-PCR, reverse transcription polymerase chain reaction; MSD, Meso Scale Discoveries; PBS, Phosphate-Buffered Saline; IL, interleukin; IFN, interferon; GM-CSF, granulocyte–macrophage colony-stimul
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; FFPE, formalin-fixed paraffin embedded; RT-PCR, reverse transcription polymerase chain reaction; FTA, Flinders Technology Associates; IgH, immunoglobulin heavy chain; qRT-PCR, quantitative reverse-transcription polymerase chain reaction; USD, United State
Concomitant partial exon skipping by a unique missense mutation of RPS6KA3 causes Coffin–Lowry syndrome
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; CLS, Coffin–Lowry syndrome; RPS6KA3, Ribosomal Protein S6 Kinase Polypeptide 3; RSK2, ribosomal S6 kinase 2; RT-PCR, reverse transcription polymerase chain reaction; cDNA, complementary DNA; ATP, adenosine triphosphate; ERK, extracellular regulated kinase
Blocking the binding of WT1 to bcl-2 promoter by G-quadruplex ligand SYUIQ-FM05
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; CD, circular dichroism; ChIP, chromatin immunoprecipitation; EMSA, electrophoretic mobility-shift assay; FRET, fluorescence resonance energy transfer; ITC, isothermal titration calorimetry; RT-PCR, reverse transcription polymerase chain reaction; FAM, 6-c
eghbx2, a homeobox gene involved in the maturation of calcified structures in Echinococcus granulosus
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; bp, Base pair(s); kDa, Kilodalton(s); RT-PCR, Reverse transcription polymerase chain reaction; Tris, Tris-(hydroxymethyl)-aminomethane; EDTA, Ethylenediamine tetraacetic acid; PBS, Phosphate buffered saline; BSA, Bovine serum albumin; TRITC, Tetramethyl r
Complete genome analysis of a rare G12P[6] rotavirus isolated in Thailand in 2012 reveals a prototype strain of DS-1-like constellation
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; RVA, species A rotaviruses; VP, viral protein; NSP, non-structural protein; BLAST, basic local alignment search tool; RT-PCR, reverse transcription polymerase chain reaction; ML, maximum likelihood; AICc, corrected Akaike information criterion; tMRCA, tim
Simvastatin induces differentiation of rabbit articular chondrocytes via the ERK-1/2 and p38 kinase pathways
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; HMG-CoA, hydroxy-methyl-glutaryl Coenzyme A; ECM, extracellular matrix; IHC, immunohistochemichemistry; IF, immunofluorescence; RT-PCR, reverse transcription polymerase chain reaction; OA, osteoarthritis; ECM, extracellular matrix; PGs, proteoglycans; BMP
Molecular cloning and characterization of an intronless farnesyl diphosphate synthase (FDP) gene from Indian rubber clone (Hevea brasiliensis Muell. Arg. RRII105): A gene involved in isoprenoid biosynthesis
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; HbFDP, Hevea brasiliensis farnesyl diphosphate synthase; PCR, Polymerase chain reaction; RT-PCR, Reverse transcription polymerase chain reaction; RRII, Rubber Research Institute of India; RRIM, Rubber Research Institute of MalaysiaCloning; Farnesyl diphos
Effects of BA and TDZ on postharvest quality and expression of laccase and aquaporin genes in cut rose ‘Sparkle’
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; RT-PCR, Reverse transcription polymerase chain reaction; TDZ, Thidiazuron; BA, Benzyladenin; PCD, Programmed cell death; AQPs, Aquaporins; TIP, Tonopelast intesinic protine; 1-MCP, 1-Methylcyclopropene; LAC, Laccase; 8-HQS, 8-Hydroxyquinoline sulfate; WU,
A novel 47.2 Mb duplication on chromosomal bands Xq21.1–25 associated with mental retardation
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; CNV, copy number variation; MR, mental retardation; aCGH, array comparative genomic hybridization; Mb, megabase; NMD, nonsense-mediated mRNA decay; OMIM, Online Mendelian Inheritance in Man; RT-PCR, reverse transcription polymerase chain reaction; XLMR, X
High-resolution 400K oligonucleotide array comparative genomic hybridization analysis of neurofibromatosis type 1-associated cutaneous neurofibromas
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; NF1, neurofibromatosis type 1; RT-PCR, reverse transcription polymerase chain reaction; BAC, bacterial artificial chromosome; CGH, comparative genomic hybridization; pNFs, plexiform neurofibromas; MPNST, malignant peripheral nerve sheath tumors; aCGH, arr
Influence of the carbamate fungicide benomyl on the gene expression and activity of aromatase in the human breast carcinoma cell line MCF-7
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; HDAC, histone deacetylase; cAMP, cyclic adenosine monophosphate; RT-PCR, reverse transcription polymerase chain reaction; FBS, fetal bovine serum; PBS, phosphate-buffered saline; ER, estrogen receptorBenomyl; Carbendazim; MCF-7; Inhibition of HDAC1; Acety
Transcriptome analysis of the plateau fish (Triplophysa dalaica): Implications for adaptation to hypoxia in fishes
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; QTP, Qinghai-Tibetan Plateau; EGLN1, Egl nine homolog 1; PPARA, peroxisome proliferator-activated receptor-a genes; EPAS1, endothelial PAS domain-containing protein 1; RT-PCR, Reverse transcription polymerase chain reaction; FPKM, Fragments Per Kilobase O
Cloning, expression, and functional characterization of the rat Pax6 5a orthologous splicing variant
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; RT-PCR, reverse transcription polymerase chain reaction; aa, amino acid; rMSCs, rat mesenchymal stem cells; MTT, methyl thiazolyl tetrazolium; PD, paired domain; NTS, NH2-terminal subdomain; CTS, COOH-terminal subdomain; P6CON, PAX6 consensus site; 5aCON,
Molecular cloning, characterization and tissue distribution of two ostrich β-defensins: AvBD2 and AvBD7
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; AvBD, avian β-defensin; RT-PCR, reverse transcription polymerase chain reaction; cDNA, DNA complementary to RNA; BLAST, basic local alignment search tool; ORFs, open reading frames; bp, base pair; Ct, threshold cycle; CFU, colony-forming unit; SPSS, stati
Characterization of the putative amino acid transporter genes AtCAT2, 3 & 4: The tonoplast localized AtCAT2 regulates soluble leaf amino acids
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; CAT, cationic amino acid transporter; TMD, transmembrane domain; RT-PCR, reverse transcription polymerase chain reaction; GUS, β-glucuronidase; GFP, green fluorescent protein; BFA, Brefeldin A; FW, fresh weight; SD, standard deviation; Arg, arginine; Gln,
Comparative biology and expression of TENP, an egg protein related to the bacterial permeability-increasing family of proteins
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; TENP, transiently expressed in neural precursors; BPI, bacterial/permeability-increasing; PLUNC, palate, lung and nasal epithelial clone; RT-PCR, reverse transcription polymerase chain reaction; LPS, lipopolysaccharide; BNF, buffered-neutral formalin; DES
Genome-wide expression analysis of rice ABC transporter family across spatio-temporal samples and in response to abiotic stresses
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; ABA, abscisic acid; ABC transporter, ATP-binding cassette transporter; As, arsenate; Cd, cadmium; COV, coefficient of variation; Cr, chromium; NBD, nucleotide binding domain; Pb, lead; RT-PCR, reverse transcription polymerase chain reaction; TMD, trans-me
Resequencing microarray method for molecular diagnosis of human arboviral diseases
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; RMA, resequencing microarray; PCR, polymerase chain reaction; RT-PCR, reverse transcription polymerase chain Reaction; PID2-RMA, PathogenID v2.0 resequencing microarrayResequencing microarray; Arboviruses; Diagnosis; Human blood samples
The over-expression of calmodulin from Antarctic notothenioid fish increases cold tolerance in tobacco
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; CaM, calmodulin; PEBV, pea early browning virus; RT-PCR, reverse transcription polymerase chain reaction; PCR, polymerase chain reaction; MDA, malondialdehyde; TBA, trichloroacetic thiobarbituric acid; TCA, thiobarbituric acid; CBF, C-repeat binding facto
Molecular cloning and functional expression of a water-soluble chlorophyll-binding protein from Japanese wild radish
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; Chl, chlorophyll; ER, endoplasmic reticulum; KTI, Kunitz-type trypsin inhibitor; RT-PCR, reverse transcription polymerase chain reaction; WSCP, water-soluble chlorophyll-binding proteinWater-soluble chlorophyll-binding protein; Class II WSCP; Kunitz-type
Alu-related transcript of TJP2 gene as a marker for colorectal cancer
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; TJP2, tight junction protein 2; RT-PCR, reverse transcription polymerase chain reaction; SINE, short interspersed nuclear element; LINE, long interspersed nuclear element; LTR, long terminal repeat; UTR, untranslated region; CDS, coding region; miRNA, mic
Expression status of mRNA for sex hormone receptors in human dental pulp cells and the response to sex hormones in the cells
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; AR, androgen receptor; ER, oestrogen receptor; RT-PCR, reverse transcription polymerase chain reaction; FBS, foetal bovine serum; HDP, human dental pulp; PBS (-), Ca2+ and Mg2+ free phosphate-buffered saline; qRT-PCR, quantitative RT-PCR; DHT, 5α-dihydrot
PLC-δ1-Lf, a novel N-terminal extended phospholipase C-δ1
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; RT-PCR, reverse transcription polymerase chain reaction; SDS–PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; cDNA, DNA complementary to RNAPI-PLC; PLC-δ1; mPLC-δ1-Lf; Phosphatidylserine; Immunohistochemistry
Association of the chicken MHC B haplotypes with resistance to avian coronavirus
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; IBV, infectious bronchitis virus; MHC, major histocompatibility complex; M41, Massachusetts 41; RT-PCR, reverse transcription polymerase chain reaction; SARS, severe acute respiratory syndrome; RSV, Rous sarcoma virus; MDV, Marek’s disease virus; SPF, spe
Isolation and functional analysis of the ClM8-FRUITFULL-like MADS-box gene from Chrysanthemum lavandulifolium
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcription polymerase chain reaction; GFP, green fluorescence protein; RFP, red fluorescence protein; M-MLV, moloney murine leukaemia virus; CaMV 35S, Cauliflower Mosaic Virus 35S; QRT-PCR, quanti
An improved method for achieving high-quality RNA for copepod transcriptomic studies
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; Na channel, sodium channel; SCG, voltage-gated sodium channel gene; RT-PCR, reverse transcription polymerase chain reaction; qPCR, quantitative PCRAcartia hudsonica; cDNA; Copepods; RNA isolation; Sodium channel; Transcriptomics
Equine rotaviruses—Current understanding and continuing challenges
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; G, glycoprotein; P, protease sensitive protein; EM, electron microscopy; ELISA, enzyme-linked immunosorbent assay; PCR, polymerase chain reaction; RT-PCR, reverse transcription polymerase chain reaction; RT-LAMP, reverse transcription loop-mediated isothe
Olive leaf down-regulates the oxidative stress and immune dysregulation in streptozotocin-induced diabetic mice
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; CAT, catalase; GPx, glutathione peroxidase; IFN, interferon; IL, interleukin; iNOS, inducible nitric oxide synthase; mRNA, messenger RNA; NO, nitric oxide; RT-PCR, reverse transcription polymerase chain reaction; SOD, superoxide dismutase; STZ, streptozot
Different matrix attachment regions flanking a transgene effectively enhance gene expression in stably transfected Chinese hamster ovary cells
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; CAT, chloramphenicol acetyltransferase; CHO, Chinese hamster ovary; ELISA, enzyme-linked immunosorbent assay; MARs, matrix attachment regions; RT-PCR, reverse transcription polymerase chain reaction; qPCR, Quantitative real-time PCRMatrix attachment regio
Microarray identification of conserved microRNAs in Pinellia pedatisecta
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; RT-PCR, reverse transcription polymerase chain reaction; qRT-PCR, Quantitative realtime polymerase chain reaction; PM, perfect match; MM, one nucleotide mismatchMicroRNA; Microarray; Pinellia pedatisecta
Identification and functional characterization of novel compound heterozygotic mutations in the TECTA gene
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; cDNA, complimentary DNA; DPOAE, distortion-product otoacoustic emissions; MT, mutant type; PCR, polymerase chain reaction; PTA, pure tone audiometry; RT-PCR, reverse transcription polymerase chain reaction; TM, tectorial membrane; vWF, von Willebrand fact
Rotavirus serotype distribution in northern Brazil trends over a 27 year period pre and post national vaccine introduction
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; UMV, Universal Mass Vaccination; MoH, Ministry of Health; PCR, polymerase chain reaction; RT-PCR, reverse transcription polymerase chain reaction; PAGE, polyacrylamide gel electrophoresis; ST, G serotypingRotavirus strains; Genotypes; Gastroenteritis; Nat
Salt-dependent increase in triterpenoids is reversible upon transfer to fresh water in mangrove plants Kandelia candel and Bruguiera gymnorrhiza
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; bASβ, -amyrin synthase; CAS, cycloartenol synthase; GC-FID, gas chromatography flame ionization detection; LUS, lupeol synthase; MS, multifunctional triterpenoid synthase; OSC, oxidosqualene cyclase; RT-PCR, reverse transcription polymerase chain reaction
Absence of SHATI/Nat8l reduces social interaction in mice
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; ANOVA, analysis of variance; BDNF, brain-derived neurotrophic factor; GDNF, glial cell line-derived neurotrophic factor; LITAF, lipopolysaccharides-induced tumor necrosis factor-α factor; RT-PCR, reverse transcription polymerase chain reaction; TNF-α, tum
The tobacco-specific carcinogen NNK induces DNA methyltransferase 1 accumulation in Laryngeal carcinoma
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; HNSCC, head and neck squamous cell carcinoma; NNK, 4-(methylnitro-samino)-1-(3-pyridyl)-1-butanone; TSGs, tumor suppressor genes; IHC, immunohistochemistry; DNMT1, DNA methyltransferase 1; RT-PCR, reverse transcription polymerase chain reaction; HPV, huma
Molecular cloning and characterization of the sheep α-TTP gene and its expression in response to different vitamin E status
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; α-TTP, α-tocopherol transfer protein; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcription polymerase chain reaction; AVED, ataxia with vitamin E deficiency; qRT-PCR, quantitative real-time polymerase chain reactionVitamin E; α-TTP; Expre
Microarray-based identification of conserved microRNAs from Pinellia ternata
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; RT-PCR, reverse transcription polymerase chain reaction; qRT-PCR, Quantitative real-time polymerase chain reaction; PM, perfect match; MM, one nucleotide mismatch; SSPE, saline–sodium phosphate–ethylenediamine tetraacetateMicroRNA; Microarray; Pinellia te
Pepsin-like aspartic protease (Sc-ASP155) cloning, molecular characterization and gene expression analysis in developmental stages of nematode Steinernema carpocapsae
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; EST, expressed sequence taq; sc-asp155, Steinernema carpocapsae aspartic protease 155; aa, amino acid; cDNA, complementary DNA; NaCl, sodium chloride; RT-PCR, reverse transcription polymerase chain reaction; RACE, rapid amplification of cDNA ends; RNA, ri
Functional complementation of dwf4 mutants of Arabidopsis by overexpression of CYP724A1
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; BR, brassinosteroid; CAPS, cleaved amplified polymorphic sequence; cM, centimorgan; EBL, 24-epibrassinolide; GA3, gibberellic acid; RT-PCR, reverse transcription polymerase chain reaction; UTR, untranslated regionBrassinosteroid metabolism; C-22 hydroxyla
Human cytosolic sialidase NEU2-low general tissue expression but involvement in PC-3 prostate cancer cell survival
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; 4MU-NeuAC, 4-methylumbelliferyl N-acetyl neuraminic acid; siRNA, small interfering RNA; RT-PCR, Reverse transcription polymerase chain reaction; SNP, single nucleotide polymorphismSialidase; NEU2 expression; Cell survival; PC-3 cells; Sp3; Runx2
Putative function of TAP63α during endochondral bone formation
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; RT-PCR, reverse transcription polymerase chain reaction; q-(RT-)PCR, quantitative or real-time (RT-) polymerase chain reaction; Gapdh, glyceraldehydes-3-phosphate dehydrogenase; Col10α1, type X collagen, alpha 1; mTrp53/63/73, murine transformation relate
Differential gene expression analysis in fracture callus of patients with regular and failed bone healing
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; RT-PCR, reverse transcription polymerase chain reaction; bp, base pairs; ECM, extracellular matrix; SLRP, small leucine-rich repeat proteoglycan; EGF, epidermal growth factor; TGFβ, transforming growth factor β; TNFα, tumor necrosis factor α; MMP, matrix
Expressed sequence tags (ESTs) based identification of genes and expression analysis of leukocyte cell-derived chemotaxin-2 (LECT2) from Epinephelus bruneus
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; ESTs, expressed sequence tags; LECT2, leukocyte cell-derived chemotaxin-2; RT-PCR, reverse transcription polymerase chain reaction; MHC, major histocompatibility complex; TLR, toll like receptor; EbLECT2, Epinephelus bruneus leukocyte cell-derived chemota
Structural Basis of Substrate Binding Specificity Revealed by the Crystal Structures of Polyamine Receptors SpuD and SpuE from Pseudomonas aeruginosa
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; ABC, ATP binding cassette; Mes, 4-morpholineethanesulfonic acid; ITC, isothermal titration calorimetry; MBP, maltodextrin binding protein; NTA, nitrilotriacetic acid; GST, glutathione S-transferase; RT-PCR, reverse transcription polymerase chain reaction;
Introduction of a novel parechovirus RT-PCR clinical test in a regional medical center
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; HPeV, human parechovirus; HEV, human enterovirus; RT-PCR, reverse transcription polymerase chain reaction; CSF, cerebrospinal fluidParechovirus; Diagnostic; PCR; Enterovirus; Encephalitis; Infant
Growth hormone can improve insulin resistance and differentiation in pancreas of senescence accelerated prone male mice (SAMP8)
Keywords: RT-PCR، واکنش زنجیره ای پلی مراز رونویسی معکوس; SAMP, the senescence-accelerated prone mouse; SAMR, senescence accelerated-resistant mouse; GH, growth hormone; IGF-1, insulin-like growth factor 1; RT-PCR, reverse transcription polymerase chain reaction; RNA, ribonucleic acid; cDNA, complementary DNA; S