Assessment of a high-throughput screening methodology for the measurement of purified UCP1 uncoupling activity Fulltext Access 6 Pages 2006
A nonradioactive, cell-free method for measuring protein synthesis inhibition by Pseudomonas exotoxin Fulltext Access 6 Pages 2006
Comparison of DNA and RNA quantification methods suitable for parameter estimation in metabolic modeling of microorganisms Fulltext Access 6 Pages 2006
Chiral resolution of the epoxyeicosatrienoic acids, arachidonic acid epoxygenase metabolites Fulltext Access 6 Pages 2006
A homogeneous, solid-phase assay for hepatitis C virus RNA-dependent RNA polymerase Fulltext Access 6 Pages 2006
Interfacing myoglobin to graphite electrode with an electrodeposited nanoporous ZnO film Fulltext Access 6 Pages 2006
Flow injection analysis-Rayleigh light scattering detection for online determination of protein in human serum sample Fulltext Access 6 Pages 2006
Voltammetric determination of mefenamic acid at lanthanum hydroxide nanowires modified carbon paste electrodes Fulltext Access 6 Pages 2006
Development of a viologen-based microtiter plate assay for the analysis of oxyanion reductase activity: Application to the membrane-bound selenate reductase from Enterobacter cloacae SLD1a-1 Fulltext Access 6 Pages 2006
High-sensitive determination of coenzyme Q10 in iodinate-β-cyclodextrin medium by inclusion reaction and catalytic polarography Fulltext Access 6 Pages 2006
Recovery and purification of highly aggregation-prone disulfide-containing peptides: Application to islet amyloid polypeptide Fulltext Access 6 Pages 2006
Enhancing the efficiency of cell-free protein synthesis through the polymerase-chain-reaction-based addition of a translation enhancer sequence and the in situ removal of the extra amino acid residues Fulltext Access 6 Pages 2006
HPLC with electrochemical detection to examine the pharmacokinetics of baicalin and baicalein in rat plasma after oral administration of a Kampo medicine Fulltext Access 6 Pages 2006
Trizol-based method for sample preparation and isoelectric focusing of halophilic proteins Fulltext Access 6 Pages 2006
A fluorescence polarization assay for screening inhibitors against the ribonuclease H activity of HIV-1 reverse transcriptase Fulltext Access 6 Pages 2006
A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays Fulltext Access 6 Pages 2006
The activity of a thermostable lipoyl synthase from Sulfolobus solfataricus with a synthetic octanoyl substrate Fulltext Access 6 Pages 2006
Fluorescence photobleaching recovery using total internal reflection interference fringes Fulltext Access 6 Pages 2006
The side chain interaction index as a tool for predicting fast-folding elements and the structure and stability of engineered peptides Fulltext Access 6 Pages 2006
Screening factors effecting a response in soluble protein expression: Formalized approach using design of experiments Fulltext Access 6 Pages 2006
Pharmacokinetics and tissue distribution of enrofloxacin and its metabolite ciprofloxacin in the Chinese mitten-handed crab, Eriocheir sinensis Fulltext Access 6 Pages 2006
Determination of daunomycin at a novel COOH/indium tin oxide ion implantation-modified electrode Fulltext Access 6 Pages 2006
A biosensor-based approach toward purification and crystallization of G protein-coupled receptors Fulltext Access 6 Pages 2006
Locked nucleic acids for optimizing displacement probes for quantitative real-time PCR Fulltext Access 6 Pages 2006
Chloroplast biogenesis 92: In situ screening for divinyl chlorophyll(ide) a reductase mutants by spectrofluorometry Fulltext Access 6 Pages 2006
Development of a high-throughput purification method and a continuous assay system for chlorophyllase Fulltext Access 6 Pages 2006
An assay for Fe(II)/2-oxoglutarate-dependent dioxygenases by enzyme-coupled detection of succinate formation Fulltext Access 6 Pages 2006
Aluminum determination in biological fluids and dialysis concentrates via chelation with 8-hydroxyquinoline and solvent extraction/fluorimetry Fulltext Access 6 Pages 2006
Identification and discrimination of extracellularly active cathepsins B and L in high-invasive melanoma cells Fulltext Access 6 Pages 2006
Evaluation of mitochondrial membrane potential using a computerized device with a tetraphenylphosphonium-selective electrode Fulltext Access 6 Pages 2006
Evaluation of saliva as a source of human DNA for population and association studies Fulltext Access 6 Pages 2006
Real-time monitoring of antibody secretion from hybridomas on a microchip by time-resolved luminescence anisotropy analysis Fulltext Access 6 Pages 2006
Molecular crowding increases the amplification success of multiple displacement amplification and short tandem repeat genotyping Fulltext Access 6 Pages 2006
Development of surface-based assays for transmembrane proteins: Selective immobilization of functional CCR5, a G protein-coupled receptor Fulltext Access 7 Pages 2006
Detection, quantification, and glycotyping of prion protein in specifically activated enzyme-linked immunosorbent assay plates Fulltext Access 7 Pages 2006
Spectrophotometric assay for calcineurin activity in leukocytes isolated from human blood Fulltext Access 7 Pages 2006
Unusual chromatographic behavior of oligonucleotide sequence isomers on two different anion exchange HPLC columns Fulltext Access 7 Pages 2006
Identification of heavy metal complexes of a hexapeptide inhibitor of the human immunodeficiency virus integrase protein by using a voltammetric approach Fulltext Access 7 Pages 2006
Quantification of dinor,dihydro metabolites of F2-isoprostanes in urine by liquid chromatography/tandem mass spectrometry Fulltext Access 7 Pages 2006
A need for caution in the use of frontal analysis continuous capillary electrophoresis for the determination of ligand binding data Fulltext Access 7 Pages 2006
Assay of the pyruvate dehydrogenase complex by coupling with recombinant chicken liver arylamine N-acetyltransferase Fulltext Access 7 Pages 2006
Blocking cardiac ATP-sensitive K+ channels reduces hydroxyl radicals caused by potassium chloride-induced depolarization in the rat myocardium Fulltext Access 7 Pages 2006
Polymerase chain reaction of 2-kb cyanobacterial gene and human anti-α1-chymotrypsin gene from genomic DNA on the In-Check single-use microfabricated silicon chip Fulltext Access 7 Pages 2006
Stimulation of polyprenyl 4-hydroxybenzoate transferase activity by sodium cholate and 3-[(cholamidopropyl)dimethylammonio]-1-propanesulfonate Fulltext Access 7 Pages 2006
Production of cis–syn thymine–thymine cyclobutane dimer oligonucleotide in the presence of acetone photosensitizer Fulltext Access 7 Pages 2006
A natural carrier effect and the generation of specific antibodies to biologically active peptides Fulltext Access 7 Pages 2006
Masking oligonucleotides improve sensitivity of mutation detection based on guanine quenching Fulltext Access 7 Pages 2006
Identification of a new chromophoric substrate in the library of amino acid p-nitroanilides for continuous assay of VanX, a d,d-dipeptidase essential for vancomycin resistance Fulltext Access 7 Pages 2006
Perfusion-culture-based secreted bioluminescence reporter assay in living cells Fulltext Access 7 Pages 2006
Whole cell immobilized amperometric biosensor based on Saccharomyces cerevisiae for selective determination of vitamin B1 (thiamine) Fulltext Access 7 Pages 2006
Determination of ofloxacin and moxifloxacin and their penetration in human aqueous and vitreous humor by using high-performance liquid chromatography fluorescence detection Fulltext Access 7 Pages 2006
Electrochemical DNA biosensor for the study of ciprofloxacin–DNA interaction Fulltext Access 7 Pages 2006
Phosphopeptides enrichment using on-line two-dimensional strong cation exchange followed by reversed-phase liquid chromatography/mass spectrometry Fulltext Access 7 Pages 2006
Automated high-throughput process for site-directed mutagenesis, production, purification, and kinetic characterization of enzymes Fulltext Access 7 Pages 2006
Negative chemical ionization gas chromatography/mass spectrometry to quantify urinary 3-methylhistidine: Application to burn injury Fulltext Access 7 Pages 2006
Support vector regression for determination of component of compound oxytetracycline powder on near-infrared spectroscopy Fulltext Access 7 Pages 2006
Application of a lanthanide fluorescent chelate label for detection of single-nucleotide mutations with peptide nucleic acid probes Fulltext Access 7 Pages 2006
Development of a high-capacity homogeneous fluorescent assay for the measurement of leukotriene B4 Fulltext Access 7 Pages 2006
Determination of dopamine in the presence of ascorbic acid by poly(styrene sulfonic acid) sodium salt/single-wall carbon nanotube film modified glassy carbon electrode Fulltext Access 7 Pages 2006
Reactions of NO2 with chromium(III) complexes with histamine and pyridoxamine ligands studied by the stopped-flow technique Fulltext Access 7 Pages 2006
An enzyme-coupled continuous spectrophotometric assay for S-adenosylmethionine-dependent methyltransferases Fulltext Access 7 Pages 2006
Nondisruptive, sequence-specific coupling of fluorochromes to plasmid DNA Fulltext Access 7 Pages 2006
Surface plasmon resonance imaging on a microchip for detection of DNA-modified gold nanoparticles deposited onto the surface in a non-cross-linking configuration Fulltext Access 7 Pages 2006
Mass spectrometric quantitation of C-reactive protein using labeled tryptic peptides Fulltext Access 7 Pages 2006
A new rapid amplification of cDNA ends method for extremely guanine plus cytosine-rich genes Fulltext Access 7 Pages 2006
A porous poly(acrylonitrile-co-acrylic acid) film-based glucose biosensor constructed by electrochemical entrapment Fulltext Access 7 Pages 2006
Application of electrochemical impedance spectroscopy for monitoring allergen–antibody reactions using gold nanoparticle-based biomolecular immobilization method Fulltext Access 7 Pages 2006
Semiautomated computer-assisted image analysis to quantify 3,3′-diaminobenzidine tetrahydrochloride-immunostained small tissues Fulltext Access 7 Pages 2006
A new colorimetric assay for methionyl aminopeptidases: Examination of the binding of a new class of pseudopeptide analog inhibitors Fulltext Access 7 Pages 2006
From purification of large amounts of phospho-compounds (nucleotides) to enrichment of phospho-peptides using anion-exchanging resin Fulltext Access 7 Pages 2006
A quantitative electrochemiluminescence assay for Clostridium perfringens alpha toxin Fulltext Access 7 Pages 2006
High-specific-activity probes and a high-resolution in-gel photo cross-linking assay for protein–DNA complexes Fulltext Access 7 Pages 2006
Lifetime fluorescence method for determining membrane topology of proteins Fulltext Access 7 Pages 2006
A nonradioisotope, enzymatic assay for 2-deoxyglucose uptake in L6 skeletal muscle cells cultured in a 96-well microplate Fulltext Access 7 Pages 2006
Affinity chromatography for purification of the modular protein growth factor receptor-bound protein 2 and development of a screening test for growth factor receptor-bound protein 2 Src homology 3 domain inhibitor using peroxidase-linked ligand Fulltext Access 7 Pages 2006
Electrochemical detection of free 3-nitrotyrosine: Application to microdialysis studies Fulltext Access 7 Pages 2006
Application of the ferrous oxidation–xylenol orange assay for the screening of 5-lipoxygenase inhibitors Fulltext Access 7 Pages 2006
A continuous fluorescence assay for phospholipase A2 with nontagged lipid Fulltext Access 7 Pages 2006
Surface plasmon resonance imaging analysis of protein–protein interactions using on-chip-expressed capture protein Fulltext Access 7 Pages 2006
A quantitative high-throughput trapping assay as a measurement of potential for bioactivation Fulltext Access 7 Pages 2006
Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder Fulltext Access 7 Pages 2006
Determination of compound aminopyrine phenacetin tablets by using artificial neural networks combined with principal components analysis Fulltext Access 7 Pages 2006
Development of an open sandwich fluoroimmunoassay based on fluorescence resonance energy transfer Fulltext Access 7 Pages 2006
A High-throughput two-phase partition assay to measure the activity of lipid-metabolizing enzymes Fulltext Access 7 Pages 2006
Structural analysis of RecA protein–DNA complexes by fluorescence-detected linear dichroism: Absence of structural change of filament for pairing of complementary DNA strands Fulltext Access 7 Pages 2006
Proton nuclear magnetic resonance spectroscopic determination of ethanol-induced formation of ethyl glucuronide in liver Fulltext Access 7 Pages 2006
Multilayer membranes for glucose biosensing via layer-by-layer assembly of multiwall carbon nanotubes and glucose oxidase Fulltext Access 7 Pages 2006
Reduced transglutaminase-catalyzed protein aggregation is observed in the presence of creatine using sedimentation velocity Fulltext Access 7 Pages 2006
Application of an enzyme chip to the microquantification of l-phenylalanine Fulltext Access 7 Pages 2006
High-throughput fluorescence polarization assay to identify small molecule inhibitors of BRCT domains of breast cancer gene 1 Fulltext Access 7 Pages 2006
Cell-surface-localized ATP detection with immobilized firefly luciferase Fulltext Access 7 Pages 2006