کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10154442 | 1666297 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polymeric stationary phases based on poly(butylene terephthalate) and poly(4-vinylpirydine) in the analysis of polyphenols using supercritical fluid chromatography. Application to bee pollen
ترجمه فارسی عنوان
فازهای ثابت پلیمری مبتنی بر پلی (بوتیل تترافاتال) و پلی (4-وینیل پرییدین) در تجزیه و تحلیل پلی فنول ها با استفاده از کروماتوگرافی مایع فوق کریتیک. کاربرد گرده زنبور عسل
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کلمات کلیدی
کروماتوگرافی مایع فوق بحرانی، فازهای مستقل مبتنی بر پلیمر، پلی فنل، گرده زنبور عسل، فلاونوئیدها، اسید فنولیک،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Two new polymer-based stationary phases; DCpak PBT (poly(butylene terephthalate)) and DCpak P4VP (poly(4-vinylpirydine)) were evaluated for the analysis of polyphenols using supercritical fluid chromatography (SFC). The compounds studied included phenolic acids and flavonoids. The different variables that influence the chromatographic separation, such as type and percentage of organic modifier, additive, pressure and temperature were examined. Using the DCpak P4VP column the retention was exceptionally high, obtaining better results with the DCpak PBT column. The separation of nine polyphenols was achieved using a gradient of modifier (methanol with 0.1% trifluoroacetic acid) from 5 to 50%, a pressure of 150â¯bar, a temperature of 35â¯Â°C and a flow-rate of 2â¯mL/min. The use of additives was necessary in order to obtain good peak shapes and efficiencies, achieving the best results with trifluoroacetic acid. LODs and LOQs values were lower than 5â¯Î¼g/mL in all the cases; meanwhile, the %RSD values for method repeatability and inter-day reproducibility were lower than 3% and 10% respectively. Finally, the proposed method was successfully applied to the analysis of polyphenols in commercial bee pollen; four compounds, namely cinnamic acid, p-coumaric acid, catechin and quercetin were identified and quantified.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1572, 19 October 2018, Pages 128-136
Journal: Journal of Chromatography A - Volume 1572, 19 October 2018, Pages 128-136
نویسندگان
Laura Toribio, Sandra Arranz, Ana M. Ares, José Bernal,