Detection of water toxicity using cytochrome P450 transgenic zebrafish as live biosensor: For polychlorinated biphenyls toxicity

► A transgenic line of zebrafish had been generated using a CYP-green fluorescence protein (CYP-GFP) construct, driven by CYP1A1 promoter. ► After exposed to PCBs, green fluorescence signals in zebrafish liver were found to express according to concentrations and time. ► The green fluorescence signal was most intense after treatment with 0.08 μg/ml PCBs. ► The maximum area of green fluorescent signal was found at 0.04 μg/ml PCBs. ► Transgenic fish with CYP-GFP is a good tool for live imaging and monitoring of aquatic contamination.