کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10529274 | 960792 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-performance liquid chromatography determination of triptolide in vitro permeation studies
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A simple, rapid and sensitive high-performance liquid chromatography (HPLC) method has been developed for the determination of triptolide. Triptolide was separated from skin endogenous and blank matrices on a 5 μm LiChrospher RP-C18 column by a mobile phase of methanol-water (65:35, v/v). The permeation samples were injected directly without pretreatment. The limit of quantitation (LOQ) and detection (LOD) for triptolide in permeation samples were far below (0.01 and 0.005 μg/mL, respectively). The method was linear over the range of 0.1-104.2 μg/mL with r2 = 0.9999. This HPLC assay is promising for measuring in vitro percutaneous penetration of triptolide through mice skins and also can be performed in the triptolide-loaded microemulsions formulation screening.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 534, Issue 2, 8 April 2005, Pages 215-221
Journal: Analytica Chimica Acta - Volume 534, Issue 2, 8 April 2005, Pages 215-221
نویسندگان
Xue-Ling Chang, Hua-Bing Chen, Xiao-Zhi Zhao, Zhong-Hong Gao, Hui-Bi Xu, Xiang-Liang Yang,