کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10559965 | 969698 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of fluoxetine and its N-desmethyl metabolite in human plasma by high-performance liquid chromatography
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A rapid and sensitive high-performance liquid chromatographic method has been developed for the simultaneous determination of the antidepressant fluoxetine and its active metabolite norfluoxetine in human plasma using paroxetine as internal standard. After liquid-liquid extraction, the compounds were separated on a C18 column using as mobile phase acetonitrile and 40Â mM potassium dihydrogen phosphate buffer (pH 2.3) in the ratio 31:69 (v/v). The quantification of fluoxetine and norfluoxetine was made by fluorescence detection at Ex/Em 230/312Â nm. The assay for each analyte was linear over the ranges 1-39 and 0.9-36Â ng/ml, respectively. For both compounds intra- and inter-day accuracy and precision ranged between â7.9-12.4 and 0.7-14.7%, respectively. The method was applied to the analysis of plasma samples obtained from healthy subjects treated with one single oral dose of 40Â mg fluoxetine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 66, Issue 3, 30 April 2005, Pages 659-663
Journal: Talanta - Volume 66, Issue 3, 30 April 2005, Pages 659-663
نویسندگان
Laurian Vlase, Silvia Imre, Sorin Leucuta,