کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10750358 | 1050298 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of silk gland ribosomes from a bivoltine caddisfly, Stenopsyche marmorata: translational suppression of a silk protein in cold conditions
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کلمات کلیدی
TORrRNADNA Data Bank of JapanDDBJN-terminal extensionNTETrichopteraCBBSDGCoomassie Brilliant Blue - Coomassie درخشان آبیRibosomal RNA - RNA RibosomalSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدRibosome - ریبوزوم، رناتنsodium dodecyl sulfate - سدیم دودسیل سولفاتtarget of rapamycin - هدف از رپامایسینTranslation control - کنترل ترجمهsucrose density gradient - گرادیان تراکم ساکارزStress granule - گرانول استرس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Larval Stenopsyche marmorata constructs food capture nets and fixed retreats underwater using self-produced proteinaceous silk fibers. In the Chikuma River (Nagano Prefecture, Japan) S. marmorata has a bivoltine life cycle; overwintering larvae grow slowly with reduced net spinning activity in winter. We recently reported constant transcript abundance of S. marmorata silk protein 1 (Smsp-1), a core S. marmorata silk fiber component, in all seasons, implying translational suppression in the silk gland during winter. Herein, we prepared and characterized silk gland ribosomes from seasonally collected S. marmorata larvae. Ribosomes from silk glands immediately frozen in liquid nitrogen (LN2) after dissection exhibited comparable translation elongation activity in spring, summer, and autumn. Conversely, silk glands obtained in winter did not contain active ribosomes and Smsp-1. Ribosomes from silk glands immersed in ice-cold physiological saline solution for approximately 4 h were translationally inactive, despite summer collection and Smsp-1 expression. The ribosomal inactivation occurs because of defects in the formation of 80S ribosomes, presumably due to splitting of 60S subunits containing 28S rRNA with central hidden break, in response to cold stress. These results suggest a novel-type ribosome-regulated translation control mechanism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 469, Issue 2, 8 January 2016, Pages 210-215
Journal: Biochemical and Biophysical Research Communications - Volume 469, Issue 2, 8 January 2016, Pages 210-215
نویسندگان
Takaomi Nomura, Miho Ito, Mai Kanamori, Yuta Shigeno, Toshio Uchiumi, Ryoichi Arai, Masuhiro Tsukada, Kimio Hirabayashi, Kousaku Ohkawa,