کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10757001 | 1050390 | 2013 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The protein kinases TPL2 and EGFR contribute to ERK1/ERK2 hyper-activation in CFTRÎF508-expressing airway epithelial cells exposed to Pseudomonas aeruginosa
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کلمات کلیدی
EGFREGFERKCFTRAECCF transmembrane regulatorMAPK - MAPKAirway epithelial cells - سلول های اپیتلیال هواییepidermal growth factor - عامل رشد اپیدرمیCystic fibrosis - فیبروز کیستیکmitogen activated protein kinase - پروتئین کیناز فعال Mitogen فعال استextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیEGF receptor - گیرنده EGF
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Excessive inflammation and Pseudomonas aeruginosa infection are two major characteristics of cystic fibrosis (CF) lung disease. In this manuscript, we describe a novel mechanism of ERK1/ERK2 activation and CXCL8 expression in airway epithelial cells (AECs) lacking functional CFTR. In both non-CF and CF AECs, the protein kinase TPL2 is required for ERK1/ERK2 MAPK activation. However, we have found that EGFR is strongly phosphorylated in the airway epithelium of CF lung and contributes to ERK1/ERK2 MAPK activation in CF AECs exposed to P. aeruginosa diffusible material (PsaDM). Moreover, PsaDM stimulates the expression of the EGFR pro-ligand HB-EGF more strongly, and in a sustained manner, in CF AECs compared to non-CF cells. Finally, although both non-CF and CF AECs expresses CXCL8 in response to PsaDM, the levels of CXCL8 are higher and EGFR plays a more important role in regulating CXCL8 synthesis in CF AECs. Together, our finding shows that in addition to the TLR-mediated TPL2 activation of ERK1/ERK2, an additional pathway contributing to ERK1/ERK2 activation is triggered by infection of CF AECs: the EGFR signaling pathway. This second pathway may contribute to excessive inflammation observed in CF.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 441, Issue 3, 22 November 2013, Pages 689-692
Journal: Biochemical and Biophysical Research Communications - Volume 441, Issue 3, 22 November 2013, Pages 689-692
نویسندگان
Guy Martel, Lucie Roussel, Simon Rousseau,