کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10768945 | 1050817 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synergic action of insulin and genistein on Na+/K+/2Cl â cotransporter in renal epithelium
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Transepithelial Clâ secretion in polarized renal A6 cells is composed of two steps: (1) Clâ entry step across the basolateral membrane mediated by Na+/K+/2Clâ cotransporter (NKCC) and (2) Clâ releasing step across the apical membrane via cystic fibrosis transmembrane conductance regulator (CFTR) Clâ channel. We estimated CFTR Clâ channel activity and transcellular Clâ secretion by measuring 5-nitro 2-(3-phenylpropylamino)benzoate (NPPB, a blocker of CFTR Clâ channel)-sensitive transepithelial conductance (Gt) and short-circuit current (Isc), respectively. Pretreatment with 1 μM insulin for 24 h had no effects on NPPB-sensitive Gt or Isc. On the other hand, in A6 cells treated with carbobenzoxy-l-leucyl-leucyl-l-leucinal (MG132; 100 μM for 2 h) that inhibits endocytosis of proteins at the plasma membrane into the cytosolic space, insulin pretreatment increased the NPPB-sensitive Isc with no effects on NPPB-sensitive Gt. Genistein (100 μM) induced sustained increases in NPPB-sensitive Gt and Isc, which were diminished by brefeldin A (a blocker of protein translocation to Golgi apparatus from endoplasmic reticulum). Co-application of insulin and genistein synergically stimulated the NPPB-sensitive Isc without any effects on NPPB-sensitive Gt. These observations suggest that: (1) insertion and endocytosis of NKCC are stimulated by insulin, (2) the insulin-induced stimulation of NKCC insertion into the basolateral membrane is offset by the stimulatory action on NKCC endocytosis from the basolateral membrane, (3) genistein stimulates insertion of both CFTR Clâ channel into the apical membrane and NKCC into the basolateral membrane, and (4) insulin and genistein synergically stimulated NKCC insertion into the basolateral membrane.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 332, Issue 4, 15 July 2005, Pages 1042-1052
Journal: Biochemical and Biophysical Research Communications - Volume 332, Issue 4, 15 July 2005, Pages 1042-1052
نویسندگان
Tomoko Ueda-Nishimura, Naomi Niisato, Hiroaki Miyazaki, Yuji Naito, Norimasa Yoshida, Toshikazu Yoshikawa, Hoyoku Nishino, Yoshinori Marunaka,