کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10799142 | 1054235 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Distinct functions of alternatively spliced isoforms encoded by zebrafish mef2ca and mef2cb
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کلمات کلیدی
qRTPCRCHDGSCAscl1apKahpfLNAMyHCMyogeninNDR1MADSNTLMEF2chordin - ChordinWISH - آرزو کردنLocked Nucleic Acid - اسید نوکلئیک قفل شدهNo tail - بدون دمTAD - بلهAlternative splicing - جابجایی جایگزینDevelopment - رشدquantitative real time PCR - زمان واقعی PCR کمیMyosin heavy chain - زنجیره سنگین میوزینhours post fertilization - ساعت پس از لقاحCMV - سیتومگالوویروسcytomegalovirus - سیتومگالوویروسSkeletal muscle - عضله اسکلتیmyocyte enhancer factor 2 - فاکتور افزایش دهنده myocyte 2BMP - مدیریت فرایند کسب و کارMYOG - منوNeurogenin 1 - نوروژنین 1Bone morphogenetic protein - پروتئین مورفوژنیک استخوانprotein kinase A - پروتئین کیناز AWhole mount in situ hybridization - کل کوهنوردی در محلkinase insert domain receptor - کیناز گیرنده دامنه را وارد کنیدZebrafish - گورخرماهیGoosecoid - گوزئکئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In mammals, an array of MEF2C proteins is generated by alternative splicing (AS), yet specific functions have not been ascribed to each isoform. Teleost fish possess two MEF2C paralogues, mef2ca and mef2cb. In zebrafish, the Mef2cs function to promote cardiomyogenic differentiation and myofibrillogenesis in nascent skeletal myofibers. We found that zebrafish mef2ca and mef2cb are alternatively spliced in the coding exons 4-6 region and these splice variants differ in their biological activity. Of the two, mef2ca is more abundantly expressed in developing skeletal muscle, its activity is tuned through zebrafish development by AS. By 24Â hpf, we found the prevalent expression of the highly active full length protein in differentiated muscle in the somites. The splicing isoform of mef2ca that lacks exon 5 (mef2ca 4-6), encodes a protein that has 50% lower transcriptional activity, and is found mainly earlier in development, before muscle differentiation. mef2ca transcripts including exon 5 (mef2ca 4-5-6) are present early in the embryo. Over-expression of this isoform alters the expression of genes involved in early dorso-ventral patterning of the embryo such as chordin, nodal related 1 and goosecoid, and induces severe developmental defects. AS of mef2cb generates a long splicing isoform in the exon 5 region (Mef2cbL) that predominates during somitogenesis. Mef2cbL contains an evolutionarily conserved domain derived from exonization of a fragment of intron 5, which confers the ability to induce ectopic muscle in mesoderm upon over-expression of the protein. Taken together, the data show that AS is a significant regulator of Mef2c activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1839, Issue 7, July 2014, Pages 559-570
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1839, Issue 7, July 2014, Pages 559-570
نویسندگان
M. Ganassi, S. Badodi, A. Polacchini, F. Baruffaldi, R. Battini, S.M. Hughes, Y. Hinits, S. Molinari,