کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10939638 | 1095282 | 2008 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characteristics of glucose uptake by glucose- and NH4-limited grown Penicillium ochrochloron at low, medium and high glucose concentration
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Glucose uptake by Penicillium ochrochloron (formerly Penicillium simplicissimum) was studied from 0.01 to 400 mM glucose using chemostat culture and bioreactor batch culture. The characteristics of glucose uptake varied considerably with the conditions of growth, harvest and uptake assay. Glucose-limited grown mycelium showed one saturable transport system [KS below 0.01 mM; vmax 1.1-1.2 mmol (g dry weight)â1 hâ1] plus a first order process (permeability P = 1.2 Ã 10â7 cm sâ1). Ammonium-limited grown mycelium showed only one saturable transport system [KS 0.3-0.7 mM; vmax 0.5-0.8 mmol (g dry weight)â1 hâ1]. During exponential growth at high glucose concentration (300-400 mM) a first order process was found with a P value of 5.6â9.3 Ã 10â7 cm sâ1. After ammonium exhaustion a second first order phase showed a lower P value (6.1-9.3 Ã 10â8 cm sâ1). A similar change in permeability was also found after a re-evaluation of published data for Gibberella fujikuroi, Aspergillus niger, Aspergillus awamori and Saccharomycopsis lipolytica. For the first order processes simple diffusion was ruled out as a mechanism for glucose uptake. Glucose uptake by P. ochrochloron was controlled more strongly by metabolism than by transport and was not rate limiting for overflow metabolism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 45, Issue 10, October 2008, Pages 1380-1392
Journal: Fungal Genetics and Biology - Volume 45, Issue 10, October 2008, Pages 1380-1392
نویسندگان
Pamela Vrabl, Wolfgang Mutschlechner, Wolfgang Burgstaller,