کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11027372 1666301 2019 21 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and validation of a method using ultra performance liquid chromatography coupled to tandem mass spectrometry for determination of zoledronic acid concentration in human bone
ترجمه فارسی عنوان
توسعه و اعتبار سنجی یک روش با استفاده از کروماتوگرافی مایع فوق العاده عملکردی همراه با طیف سنجی جرم دو طرفه برای تعیین غلظت اسید زولدرونیک در استخوان انسان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A method for the extraction and quantification of zoledronic acid (ZA) from human bone was set up and validated. This method allowed the quantification of ZA from jawbone sequestrations of patients affected by bisphosphonate-related osteonecrosis of the jaw (BRONJ) associated with ZA treatment. The analyte was extracted from the bone tissues with phosphoric acid and derivatized using trimethylsilyl diazomethane (TMS-DAM). ZA tetramethyl phosphonate was then quantified by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), showing high accuracy, repeatability and selectivity. Lower limits of quantification and detection (LLOQ and LLQD) were 3.4 ng/mL and 1 ng/mg, respectively. This study fully described the analytical process for the determination of ZA in human bone sequestrations, representing a pivotal step for further biomedical research on ZA and BRONJ.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 162, 5 January 2019, Pages 286-290
نویسندگان
, , , , , , , ,