کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11031875 | 1645770 | 2018 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro and in vivo evaluation of the genotoxicity of Eriobotrya japonica leaf extract
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کلمات کلیدی
FBSChlNCEOECDMFDSMinistry of Food and Drug SafetyChromosome aberration test - آزمون انحراف کروموزومیBacterial reverse mutation test - آزمون جهش معکوس باکتریاییMicronucleus test - آزمون میکرونوکلئوسnormochromatic erythrocyte - اریتروسیت نورموشروماتیکpolychromatic erythrocyte - اریتروسیت چند رنگیOrganisation for Economic Cooperation and Development - سازمان همکاری اقتصادی و توسعهfetal bovine serum - سرم جنین گاوGenotoxicity - سمیت ژنتیکی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Eriobotrya japonica leaf is included in the Chinese Pharmacopoeia, and is widely used as a medicinal material in traditional medicine. The present study investigated the potential genotoxic effects of E. japonica leaf extract (EJE) using three standard battery systems. Genotoxicity tests were conducted following the test guidelines of the Organisation for Economic Cooperation and Development (OECD) and Ministry of Food and Drug Safety (MFDS), with application of Good Laboratory Practice. The bacterial reverse mutation test was conducted using the pre-incubation method in the presence or absence of the metabolic activation system (S9 mixture). The in vitro chromosome aberration test was performed using cultured Chinese hamster lung cell line in the presence or absence of the S9 mixture. The in vivo micronucleus test was performed using ICR mice. The bacterial reverse mutation test with Salmonella typhimurium strains TA98, TA100, TA1535, and TA1537 and Escherichia coli strain WP2uvrA showed that EJE did not induce gene mutations at any dose level in all the strains tested. EJE also did not show any chromosomal aberrations in the in vitro chromosomal aberration test and in the in vivo micronucleus test. These results showed that EJE did not induce mutagenicity or clastogenicity in either in vitro or in vivo systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Toxicology and Pharmacology - Volume 99, November 2018, Pages 238-243
Journal: Regulatory Toxicology and Pharmacology - Volume 99, November 2018, Pages 238-243
نویسندگان
Myung-Hwan Jeong, Nak-Won Seong, Jong-Yun Lee, Yong-Jae Kim, Na-Rae Shin, Jong-Choon Kim,