کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1162989 1490914 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An ultrasensitive lysozyme chemiluminescence biosensor based on surface molecular imprinting using ionic liquid modified magnetic graphene oxide/β-cyclodextrin as supporting material
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
An ultrasensitive lysozyme chemiluminescence biosensor based on surface molecular imprinting using ionic liquid modified magnetic graphene oxide/β-cyclodextrin as supporting material
چکیده انگلیسی


• A CL biosensor for Lys was obtained coupled with SMIP based on ILs-Fe3O4@DA/GO/β-CD.
• Absorption capacity of ILs-Fe3O4@DA/GO/β-CD-SMIP to Lys reached up to 101 mg/g.
• The adsorption equilibrium was reached within 10 min.
• Adsorption model followed Langmuir isotherm.
• The CL system was luminol, K3[Fe(CN)6] and NaOH solutions.

In this work, ionic liquid modified Fe3O4@dopamine/graphene oxide/β-cyclodextrin (ILs-Fe3O4@DA/GO/β-CD) was used as supporting material to synthesize surface molecularly imprinted polymer (SMIP) which then was introduced into chemiluminescence (CL) to achieve an ultrasensitive and selective biosensor for determination of lysozyme (Lys). ILs and β-CD was applied to provide multiple binding sites to prepare Lys SMIP and Fe3O4@DA was designed to make the product separate easily and prevent the aggregation of GO which could improve absorption capacity for its large specific surface area. The ILs-Fe3O4@DA/GO/β-CD-SMIP showed high adsorption capacity (Q = 101 mg/g) to Lys in the adsorption isotherm assays. The adsorption equilibrium was reached within 10 min for all the concentrations, attributing to the binding sites situated exclusively at the surface, and the adsorption model followed Langmuir isotherm. Under the suitable CL conditions, the proposed biosensor could response Lys linearly in the range of 1.0 × 10−9–8.0 × 10−8 mg/mL with a detection limit of 3.0 × 10−10 mg/mL. When used in practical samples in determination of Lys, the efficient biosensor exhibited excellent result with the recoveries ranging from 94% to 112%.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 918, 28 April 2016, Pages 89–96
نویسندگان
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