کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1170633 | 1491179 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid screening of pathogenic bacteria using solid phase concentration and diffuse reflectance spectroscopy
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A new methodology to rapidly screen for pathogenic bacteria in various liquids (e.g., potable water and juice) is described. It combines the selectivity of dye-labeled antibodies, the sample concentration capability of solid phase membrane filtration, and the facile readout of the concentrated, dye-labeled microorganisms by diffuse reflectance spectroscopy (DRS). Details about the selection of the most effective membrane filter, detection of target bacteria in different types of liquids, and evaluation of assay specificity in screening for E. coli O157:H7 are discussed. For this pathogen, the technique has a working range of 5Â ÃÂ 105 to 5Â ÃÂ 108Â cells/mL and an overall work up time of â¼45Â min. The amount of captured bacteria is directly determined in only 2Â s by using a hand-held DRS instrument via comparisons to a calibration curve based on the Kubelka-Munk function. Overall, this assay system offers high speed, simplicity, and low cost, making it a potential alternative for screening of several types of bacterial contaminated samples in almost any location.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 569, Issues 1â2, 31 May 2006, Pages 83-90
Journal: Analytica Chimica Acta - Volume 569, Issues 1â2, 31 May 2006, Pages 83-90
نویسندگان
Salma Rahman, Robert J. Lipert, Marc D. Porter,